期刊
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
卷 325, 期 1, 页码 H113-H124出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.00036.2023
关键词
aneurysm; ascending thoracic aortic aneurysm; convection; diffusion; extracellular matrix; transport
Thoracic aortic aneurysm is characterized by dilation of the aortic diameter by greater than 50%, which can lead to dissection or rupture. This study found that aneurysm severity in the ascending thoracic aorta is associated with changes in mass transport parameters and extracellular matrix organization. The results suggest that an initial increase in solute permeability is followed by a decrease in hydraulic conductance after the aneurysm has formed. Elastic fiber fragmentation is also observed in the extracellular matrix, which could contribute to aneurysm progression or treatment options.
Thoracic aortic aneurysm is characterized by dilation of the aortic diameter by greater than 50%, which can lead to dissection or rupture. Common histopathology includes extracellular matrix remodeling that may affect transmural mass transport, defined as the movement of fluids and solutes across the wall. We measured in vitro ascending thoracic aorta mass transport in a mouse model with partial aneurysm phenotype penetration due to a mutation in the extracellular matrix protein fibulin-4 [Fbln4E57K/E57K, referred to as MU-A (aneurysm) or MU-NA (no aneurysm)]. To push the aneurysm phenotype, we also included MU mice with reduced levels of lysyl oxidase [Fbln4(E57K/E57K);Lox(+/-), referred to as MU-XA (extreme aneurysm)] and compared all groups to wild-type (WT) littermates. The phenotype variation allows investigation of how aneurysm severity correlates with mass transport parameters and extracellular matrix organization. We found that MU-NA ascending thoracic aortae have similar hydraulic conductance (Lp) to WT, but 397% higher solute permeability (x) for 4 kDa FITC-dextran. In contrast, MU-A and MU-XA ascending thoracic aortae have 44-68% lower Lp and similar x to WT. The results suggest that ascending thoracic aortic aneurysm progression involves an initial increase in x, followed by a decrease in Lp after the aneurysm has formed. All MU ascending thoracic aortae are longer and have increased elastic fiber fragmentation in the extracellular matrix. There is a negative correlation between diameter and Lp or x in MU ascending thoracic aortae. Changes in mass transport due to elastic fiber fragmentation could contribute to aneurysm progression or be leveraged for treatment.
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