Reinforcing Tissue-Engineered Cartilage: Nanofibrillated Cellulose Enhances Mechanical Properties of Alginate Dialdehyde-Gelatin Hydrogel

Cartilage tissue engineering offers a promising option for treating osteochondral defects. Alginate dialdehyde-gelatin (ADA-GEL) hydrogel has been explored as promising material for soft tissue scaffolds; however, its low stiffness has posed a constraint to load bearing applications. Herein, this limitation is addressed by introducing nanofibrillated cellulose (NFC) into the ADA-GEL matrix. The effect of NFC on the physicochemical properties of hydrogels is evaluated. Fourier transform infrared spectra demonstrate no chemical interaction between NFC and ADA-GEL, while scanning electron microscopy pictures reveal NFC fibers embedded in the hydrogel matrix, thus confirming the fiber-reinforced composite hypothesis. NFC-reinforced ADA-GEL (AG-N) composite hydrogels exhibit increased stiffness, with a maximum compressive effective modulus of 19.6 & PLUSMN; 3.0 kPa at 25% w/w NFC content. ATDC5 cell viability and proliferation as well as chondrogenic differentiation are assessed using immunohistochemical staining for sulfated glycosaminoglycans and collagen type II. A possible application of AG-N hydrogels as an osteochondral plug is also proposed, with polyetheretherketone as the subchondral bone anchor part. The mechanical properties of the resulting osteochondral device highlight its potential as a promising biomaterial for treatment of osteochondral defects. These findings provide valuable insights into the development of AG-N hydrogels for load-bearing tissue engineering applications. Nanofibrillated cellulose (NFC) is added as a reinforcing phase to alginate dialdehyde-gelatin for developing hydrogels for cartilage repair. Composite hydrogels exhibit increased stiffness, e.g., 19.6 & PLUSMN; 3.0 kPa. ATDC5 cell viability, proliferation, and chondrogenic differentiation are assessed using immunohistochemical staining for sulfated glycosaminoglycans and collagen type II. An osteochondral plug is designed with polyetheretherketone as the subchondral bone anchor part.image & COPY; 2023 WILEY-VCH GmbH

Automated summary

Cartilage tissue engineering is improved by adding nanofibrillated cellulose (NFC) to alginate dialdehyde-gelatin (ADA-GEL) hydrogel, resulting in increased stiffness of the composite hydrogel. The maximum compressive effective modulus is 19.6 kPa. Cell viability, proliferation, and chondrogenic differentiation of ATDC5 cells are evaluated using immunohistochemical staining. The potential application of an osteochondral plug with polyetheretherketone as the subchondral bone anchor part is proposed.