Application of super-resolution microscopy in mitochondria-dynamic diseases

Limited by spatial and temporal resolution, traditional optical microscopy cannot image the delicate ultrastructure organelles and sub-organelles. The emergence of super-resolution microscopy makes it possible. In this review, we focus on mitochondria. We summarize the process of mitochondrial dynamics, the primary proteins that regulate mitochondrial morphology, the diseases related to mitochondrial dynamics. The purpose is to apply super-resolution microscopy developed during recent years to the mitochondrial research. By providing the right research tools, we will help to promote the application of this technique to the in-depth elucidation of the pathogenesis of diseases related to mitochondrial dynamics, assistdiagnosis and develop the therapeutic treatment.

Automated summary

Traditional optical microscopy is limited in imaging delicate organelles and sub-organelles due to its spatial and temporal resolution. The emergence of super-resolution microscopy has made it possible to overcome these limitations. This review focuses on mitochondria and summarizes the dynamics, morphology-regulating proteins, and diseases associated with mitochondrial dynamics. By applying super-resolution microscopy, this technique can be used to explore the pathogenesis of these diseases, aid in diagnosis, and develop therapeutic treatments.