期刊
MEAT SCIENCE
卷 115, 期 -, 页码 34-40出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.meatsci.2016.01.003
关键词
Calcium imbalance; Mitochondrial; Apoptosis; PSE meat
资金
- Twelfth Five-Year National Science and Technology Support Project [2012BAD28B01]
Eight pale, soft and exudative (PSE) and eight reddish-pink, firm and non-exudative (RFN) porcine longissimus muscle samples were selected based on pH and 1* at 1 h postmortem (PM), and drip loss at 24 h PM, and used to evaluate the cellular calcium and apoptosis status. We found that SERCA1 was decreased, while IP3R was decreased in PSE meat (P < 0.05), indicative of the overloaded sarcoplasmic calcium status. In PSE meat, the pro-apoptotic factor BAX was increased while the anti-apoptotic factor Bcl-2 was decreased (P < 0.05). The significantly increased activity of caspase 3 and the expression of its cleavage fragment suggested higher apoptotic potential in PSE meat compared with RFN meat (P < 0.05). Moreover, the significantly higher expression level of cytochrome C (P < 0.05) suggests the important role of mitochondria during apoptosis appearance in PSE meat Taken together, our data inferred that the calcium channel disorder present in PSE meat was associated with the increased apoptotic potential. (C) 2016 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据