4.6 Article

Site-Specific Introduction of Alanines for the Nuclear Magnetic Resonance Investigation of Low-Complexity Regions and Large Biomolecular Assemblies

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Biochemistry & Molecular Biology

Multi-site-specific isotopic labeling accelerates high-resolution structural investigations of pathogenic huntingtin exon-1

Carlos A. Elena-Real et al.

Summary: Huntington's disease neurodegeneration occurs when the number of consecutive glutamines in the hunting -tin exon-1 (HTTExon1) exceeds a pathological threshold of 35. The sequence homogeneity of HTTExon1 reduces the signal dispersion in NMR spectra, hampering its structural characterization. The proposed strategy of introducing isotopically labeled glutamines in a site-specific manner allows for the study of high-resolution structure and function in low-complexity regions.

STRUCTURE (2023)

Article Chemistry, Multidisciplinary

Deciphering how naturally occurring sequence features impact the phase behaviours of disordered prion-like domains

Anne Bremer et al.

Summary: This study uncovers how evolutionarily conserved compositional biases influence the phase behavior of Prion-like low-complexity domains (PLCDs). Tyrosine is identified as a stronger sticker, while lysine weakens sticker-sticker interactions. Additionally, glycine and serine residues act as non-equivalent spacers, impacting the driving forces for phase separation in PLCDs.

NATURE CHEMISTRY (2022)

Review Chemistry, Multidisciplinary

NMR Provides Unique Insight into the Functional Dynamics and Interactions of Intrinsically Disordered Proteins

Aldo R. Camacho-Zarco et al.

Summary: This review introduces the applications of nuclear magnetic resonance (NMR) in understanding the structure, dynamic behavior, and interaction trajectories of intrinsically disordered proteins. NMR provides ensemble averaged structural and dynamic parameters for each assigned resonance, revealing the importance of these parameters in the kinetics and thermodynamics of cellular and extracellular reactions. Furthermore, NMR can uncover the mechanistic basis of functional disordered molecular assemblies that are crucial for human health.

CHEMICAL REVIEWS (2022)

Article Biochemical Research Methods

The sequence context in poly-alanine regions: structure, function and conservation

Pablo Mier et al.

Summary: This study investigates the structure and function of poly-alanine regions in proteins, identifying glycine and proline as the most abundant amino acids within polyA and its flanking regions, which influence the α-helical conformations predicted for polyA. Additionally, the research reveals the relationship between the position of polyA in protein N-termini and their function as transit peptides.

BIOINFORMATICS (2022)

Review Chemistry, Multidisciplinary

NMR Provides Unique Insight into the Functional Dynamics and Interactions of Intrinsically Disordered Proteins

Aldo R. Camacho-Zarco et al.

Summary: Intrinsically disordered proteins play essential roles in cellular and extracellular biochemistry. Nuclear magnetic resonance is a powerful tool for studying their structural and dynamic behavior, providing insights into reaction kinetics and thermodynamics essential for function. Recent applications of NMR have helped uncover the mechanistic basis of functional disordered molecular assemblies important for human health.

CHEMICAL REVIEWS (2022)

Article Biochemistry & Molecular Biology

Developments in solution-state NMR yield broader and deeper views of the dynamic ensembles of nucleic acids

Bei Liu et al.

Summary: Recent advances in solution-state nuclear magnetic resonance (NMR) methods, integrated with computational techniques, have improved the ability to probe the dynamic ensembles of DNA and RNA, leading to new mechanistic insights into gene expression and regulation.

CURRENT OPINION IN STRUCTURAL BIOLOGY (2021)

Review Chemistry, Physical

Methyl TROSY spectroscopy: A versatile NMR approach to study challenging biological systems

Stefan Schuetz et al.

PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY (2020)

Article Biochemistry & Molecular Biology

Unblending of Transcriptional Condensates in Human Repeat Expansion Disease

Shaon Basu et al.

Article Biochemistry & Molecular Biology

Musashi-1: An Example of How Polyalanine Tracts Contribute to Self-Association in the Intrinsically Disordered Regions of RNA-Binding Proteins

Tsai-Chen Chen et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

Review Biochemistry & Molecular Biology

Prion-like low-complexity sequences: Key regulators of protein solubility and phase behavior

Titus M. Franzmann et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2019)

Article Chemistry, Multidisciplinary

A General Strategy to Access Structural Information at Atomic Resolution in Polyglutamine Homorepeats

Annika Urbanek et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018)

Article Biochemistry & Molecular Biology

Late metabolic precursors for selective aromatic residue labeling

Julia Schoerghuber et al.

JOURNAL OF BIOMOLECULAR NMR (2018)

Article Biochemistry & Molecular Biology

POTENCI: prediction of temperature, neighbor and pH-corrected chemical shifts for intrinsically disordered proteins

Jakob Toudahl Nielsen et al.

JOURNAL OF BIOMOLECULAR NMR (2018)

Article Multidisciplinary Sciences

Reversible inhibition of the ClpP protease via an N-terminal conformational switch

Siavash Vahidi et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2018)

Article Multidisciplinary Sciences

Distinct ways of G:U recognition by conserved tRNA binding motifs

Yeeting E. Chong et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2018)

Article Chemistry, Multidisciplinary

XLSY: Extra-Large NMR Spectroscopy

Yulia Pustovalova et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018)

Article Chemistry, Physical

Injectable tissue integrating networks from recombinant polypeptides with tunable order

Stefan Roberts et al.

NATURE MATERIALS (2018)

Review Biochemistry & Molecular Biology

Intrinsic Disorder in Proteins with Pathogenic Repeat Expansions

April L. Darling et al.

MOLECULES (2017)

Review Microbiology

Mechanistic insights into bacterial AAA plus proteases and protein-remodelling machines

Adrian O. Olivares et al.

NATURE REVIEWS MICROBIOLOGY (2016)

Article Biochemistry & Molecular Biology

Methyl-specific isotopic labeling: a molecular tool box for solution NMR studies of large proteins

Rime Kerfah et al.

CURRENT OPINION IN STRUCTURAL BIOLOGY (2015)

Article Biochemistry & Molecular Biology

Polyalanine expansions drive a shift into α-helical clusters without amyloid-fibril formation

Saskia Polling et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2015)

Article Biochemistry & Molecular Biology

Polyalanine expansions drive a shift into α-helical clusters without amyloid-fibril formation

Saskia Polling et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2015)

Review Biochemistry & Molecular Biology

Bringing Dynamic Molecular Machines into Focus by Methyl-TROSY NMR

Rina Rosenzweig et al.

ANNUAL REVIEW OF BIOCHEMISTRY, VOL 83 (2014)

Article Multidisciplinary Sciences

The structure of the box C/D enzyme reveals regulation of RNA methylation

Audrone Lapinaite et al.

NATURE (2013)

Review Physiology

Congenital central hypoventilation syndrome

N. Ramanantsoa et al.

RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY (2013)

Article Multidisciplinary Sciences

Unraveling the Mechanism of Protein Disaggregation Through a ClpB-DnaK Interaction

Rina Rosenzweig et al.

SCIENCE (2013)

Article Chemistry, Multidisciplinary

Multiple-Site Labeling of Proteins with Unnatural Amino Acids

Karin V. Loscha et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2012)

Review Biochemistry & Molecular Biology

ClpXP, an ATP-powered unfolding and protein-degradation machine

Tania A. Baker et al.

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH (2012)

Article Biochemistry & Molecular Biology

A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies

Carlos Amero et al.

JOURNAL OF BIOMOLECULAR NMR (2011)

Article Biochemical Research Methods

Flexizymes for genetic code reprogramming

Yuki Goto et al.

NATURE PROTOCOLS (2011)

Article Chemistry, Multidisciplinary

Automatic Assignment of the Intrinsically Disordered Protein Tau with 441-Residues

Rhagavendran L. Narayanan et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2010)

Article Chemistry, Multidisciplinary

Fast Two-Dimensional NMR Spectroscopy of High Molecular Weight Protein Assemblies

Carlos Amero et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2009)

Article Multidisciplinary Sciences

The structure of alanyl-tRNA synthetase with editing domain

Masaaki Sokabe et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2009)

Review Chemistry, Physical

Stable isotope labeling methods for protein NMR spectroscopy

Shin-Ya Ohki et al.

PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY (2008)

Article Biochemistry & Molecular Biology

Correlation between 13Cα chemical shifts and helix content of peptide ensembles

Daniel S. Weinstock et al.

PROTEIN SCIENCE (2008)

Article Multidisciplinary Sciences

Quantitative dynamics and binding studies of the 20S proteasome by NMR

Remco Sprangers et al.

NATURE (2007)

Article Biochemical Research Methods

A highly flexible tRNA acylation method for non-natural potypeptide synthesis

H Murakami et al.

NATURE METHODS (2006)

Article Biochemical Research Methods

Isotope labeling strategies for the study of high-molecular-weight proteins by solution NMR spectroscopy

Vitali Tugarinov et al.

NATURE PROTOCOLS (2006)

Article Multidisciplinary Sciences

Molecular basis of alanine discrimination in editing site

M Sokabe et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2005)

Article Biochemistry & Molecular Biology

The CCPN data model for NMR spectroscopy: Development of a software pipeline

WF Vranken et al.

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS (2005)

Article Chemistry, Multidisciplinary

Water-solubilized, cap-stabilized, helical polyalanines: Calibration standards for NMR and CD analyses

B Heitmann et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2005)

Review Biochemistry & Molecular Biology

Polyalanine expansions in human

J Amiel et al.

HUMAN MOLECULAR GENETICS (2004)

Article Multidisciplinary Sciences

The polyproline II conformation in short alanine peptides is noncooperative

K Chen et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2004)

Article Biochemistry & Molecular Biology

Polymorphism, shared functions and convergent evolution of genes with sequences coding for polyalanine domains

H Lavoie et al.

HUMAN MOLECULAR GENETICS (2003)

Article Multidisciplinary Sciences

Polyproline II structure in a sequence of seven alanine residues

ZS Shi et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2002)