4.8 Article

Orientation-Controllable Enzyme Cascade on Electrode for Bioelectrocatalytic Chain Reaction

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Chemistry, Analytical

3D DNA walker recognition-driven homogeneous dual-mode sensing strategy based on enzyme biofuel cell for ultrasensitive detection of HER2

Yang-Yang Hou et al.

Summary: An ultrasensitive enzyme biofuel cell (EBFC) based self-powered biosensor is developed for dual-mode detection of HER2. Graphdiyne (GDY) and tetragonal molybdenum disulfide (1T MoS2) are used to construct high-performance EBFC. Threedimensional (3D) DNA Walker triggers hybrid chain reaction (HCR) cascade strategy is employed to improve the assay's output signal intensity and selectivity. Additionally, a capacitor is matched to EBFC for magnifying the electrical signals and boosting the analytical performance. The developed ultrasensitive dual-mode assay exhibits an expanded linear response range and low detection limit for HER2, providing a powerful tool for accurate detection of cancer marker.

SENSORS AND ACTUATORS B-CHEMICAL (2023)

Article Nanoscience & Nanotechnology

Co-Immobilization of Enzymes and Metals on the Covalent-Organic Framework for the Efficient Removal of Mycotoxins

Caicai Fu et al.

Summary: In this study, a chemoenzymatic cascade reaction using a hybrid catalyst is proposed for the efficient and green removal of mycotoxins. The GOx-Fe3O4@COF hybrid catalyst, synthesized by compartmental co-immobilized glucose oxidase (GOx) and Fe3O4 nanoparticles (NPs) on a flower-shaped covalent organic framework (COF), exhibits excellent activity in mycotoxin removal. The degradation efficiency of aflatoxin B-1 (AFB(1)) is significantly higher in the chemoenzymatic cascade reaction, catalyzed by GOx-Fe3O4@COF, compared to the Fenton reaction catalyzed by Fe3O4@COF. The GOx-Fe3O4@COF hybrid catalyst shows high activity in a wide pH range of 3.0-7.0, overcoming the pH limitation of the Fenton reaction.

ACS APPLIED MATERIALS & INTERFACES (2023)

Review Chemistry, Multidisciplinary

From Protein Film Electrochemistry to Nanoconfined Enzyme Cascades and the Electrochemical Leaf

Fraser A. Armstrong et al.

Summary: Protein film electrochemistry (PFE) provides valuable insights into the properties and behaviors of redox proteins and electron-transferring enzymes, allowing the study of previously unclear aspects such as unstable Fe-S centers and enzyme catalytic biases. By attaching enzymes to electrodes, their reversible electrocatalytic capabilities have been revealed, and interesting similarities between catalytic rates and electronics have been observed. One particular case involves the reversible electrochemistry of a photosynthetic enzyme, ferredoxin-NADP+ reductase (FNR), which has led to the development of a new technology called cascade-tronics, where reactions in enzyme cascades can be monitored and controlled using an electrochemical analyzer. The electrochemical leaf (e-Leaf) offers a natural way to study enzyme behaviors under nanoconfinement and crowding, mimicking the conditions in living cells.

CHEMICAL REVIEWS (2023)

Article Nanoscience & Nanotechnology

Selective Coimmobilization of His-Tagged Enzymes on Yttrium-Stabilized Zirconia-Based Membranes for Continuous Asymmetric Bioreductions

Daniel Andres-Sanz et al.

Summary: In this study, a novel interaction between YSZ materials and His-tagged enzymes was discovered, enabling the fabrication of multifunctional biocatalytic membranes for continuous biomanufacturing. This system succeeded in in situ redox cofactor recycling, maintaining specific productivity with minimal NADH usage, and accumulating a high enzyme turnover number in a 24-hour period.

ACS APPLIED MATERIALS & INTERFACES (2022)

Article Chemistry, Multidisciplinary

Integration of a capacitor to a 3-D DNA walker and a biofuel cell-based self-powered system for ultrasensitive bioassays of microRNAs

Fu-Ting Wang et al.

Summary: The self-powered microRNA biosensor with triple signal amplification systems integrates DNA walkers, enzymatic biofuel cells, and a capacitor to achieve high-performance bioanalysis and has good potential for clinical applications.

NANOSCALE (2022)

Review Chemistry, Physical

Electron transfer-driven single and multi-enzyme biofuel cells for self-powering and energy bioscience

Saikat Dutta et al.

Summary: Single enzyme fuel cells have the unique capability of degrading organics and generating electricity. Factors such as bioelectrode fabrication, enzyme conformation, and electron transport mechanism significantly influence the power density. These fuel cells have advanced applications in areas such as waste-water treatment, energy storage, and self-powering biosensors.

NANO ENERGY (2022)

Article Chemistry, Physical

3D-printed hierarchical pillar array electrodes for high-performance semi-artificial photosynthesis

Xiaolong Chen et al.

Summary: The use of aerosol jet printing with indium tin oxide nanoparticles allows for the generation of hierarchical electrode structures that meet the complex requirements for high biophotoelectrochemical performance. The resulting micropillar array electrodes, when wired to cyanobacteria, exhibit significantly improved biocatalyst loading, light utilization, and electron flux output. This study demonstrates a promising approach to more efficiently harnessing bio-energy from photosynthesis and provides new tools for three-dimensional electrode design.

NATURE MATERIALS (2022)

Article Electrochemistry

Direct electrical contact of NAD+/NADH-dependent dehydrogenase on electrode surface enabled by non-native solid-binding peptide as a molecular binder

Stacy Simai Reginald et al.

Summary: This study introduces a gold-binding peptide to NAD(+)/NADH-dependent formate dehydrogenase (FDH) through genetic modification, enabling direct wiring of the enzyme to the electrode surface. The gold-binding activity of the fused enzymes depends on fusion site and tertiary structure, with the highest activity observed at the C-terminus. The presence of the gold-binding peptide increases the stability of the enzyme electrode and promotes direct electrochemical reactions. This method shows promise for bio-electronic applications involving NAD(+)/NADH-dependent enzymes.

ELECTROCHIMICA ACTA (2022)

Article Chemistry, Multidisciplinary

Engineering Electro- and Photocatalytic Carbon Materials for CO2 Reduction by Formate Dehydrogenase

Vivek M. Badiani et al.

Summary: Semiartificial approaches integrating enzymes with synthetic materials can efficiently produce renewable fuels. Positive-charged carbon nanotubes (CNTs) were found to effectively immobilize the CO2 reductase, achieving efficient electrochemical CO2 reduction. Furthermore, coupling the enzyme with positive-charged amorphous carbon dots (a-CDs) resulted in high rates of photocatalytic CO2 reduction.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2022)

Article Chemistry, Physical

A Nanoconfined Four-Enzyme Cascade Simultaneously Driven by Electrical and Chemical Energy, with Built-in Rapid, Confocal Recycling of NADP(H) and ATP

Clare F. Megarity et al.

Summary: The importance of energized nanoconfinement in facilitating the study and execution of enzyme cascades with multiple exchangeable cofactors is demonstrated through experiments with carboxylic acid reductase.

ACS CATALYSIS (2022)

Article Chemistry, Multidisciplinary

Recent Advances in Signal Amplification to Improve Electrochemical Biosensing for Infectious Diseases

Xingcheng Zhou et al.

Summary: In the field of infectious disease diagnostics, access to healthcare resources is unequal. Point-of-care (POC) diagnostics, especially those that can be used in non-laboratory settings, are appealing for disease control in resource-limited environments. To improve the sensitivity and specificity, signal amplification strategies have been developed for electrochemical biosensors. This perspective reviews recent advances in signal amplification strategies, specifically for biosensors that detect nucleic acids and pathogenic microbes. These strategies are classified into target-based amplification and signal-based amplification. The authors also emphasize the importance of designing signal amplification strategies that are tailored to the application location and disease target, and that take into account the resources required for production and operation of the sensor, particularly when the sensor is intended for use in low-resource settings. It is anticipated that incorporating signal-based amplification strategies will expand the usage of electrochemical POC devices for diagnosing infectious diseases in various settings.

FRONTIERS IN CHEMISTRY (2022)

Article Nanoscience & Nanotechnology

Synthetic Protocell as Efficient Bioreactor: Enzymatic Superactivity and Ultrasensitive Glucose Sensing in Urine

Tushar Kanti Mukherjee et al.

Summary: In this study, a high-performance integrated cascade bioplatform based on synthetic droplets was developed for ultrasensitive glucose sensing. The fabrication of liquidlike membraneless droplets using multivalent electrostatic interactions was reported, demonstrating the remarkable enhancement in enzymatic activity inside the droplets. The composite platform showed high encapsulation efficiency, stability, and ultrasensitive glucose sensing capability.

ACS APPLIED MATERIALS & INTERFACES (2022)

Article Chemistry, Analytical

Real-Time Biosensor Platform Based on Novel Sandwich Graphdiyne for Ultrasensitive Detection of Tumor Marker

Jing Xu et al.

Summary: Realization of a highly sensitive analysis and sensing platform is crucial for early-stage tumor diagnosis. In this study, a self-powered biosensor with a novel sandwich graphdiyne (SGDY) structure combined with an aptamer-specific recognition function was developed to sensitively and accurately detect tumor markers.

ANALYTICAL CHEMISTRY (2022)

Article Chemistry, Analytical

Construction of an Integrated Device of a Self-Powered Biosensor and Matching Capacitor Based on Graphdiyne and Multiple Signal Amplification: Ultrasensitive Method for MicroRNA Detection

Yang-Yang Hou et al.

Summary: A novel self-powered biosensing platform was developed for detecting miRNA, utilizing an EBFC-based biosensor with a matching capacitor. By improving the EBFC performance with catalytic hairpin assembly and hybrid chain reaction, along with using a matching capacitor for signal amplification, the sensitivity was significantly increased.

ANALYTICAL CHEMISTRY (2021)

Article Biochemistry & Molecular Biology

Neutron crystallography for the elucidation of enzyme catalysis

Fumiaki Kono et al.

Summary: Neutron crystallography plays a crucial role in structural biology by directly observing the positions and roles of hydrogen atoms in proteins. Recent studies have used neutron crystallography to analyze the structures of three enzymes, revealing their important roles in molecular recognition and catalytic reactions.

CURRENT OPINION IN STRUCTURAL BIOLOGY (2021)

Article Biophysics

Versatile electrochemical biosensor based on bi-enzyme cascade biocatalysis spatially regulated by DNA architecture

Yongcun Yan et al.

Summary: A one-pot method based on RCA is developed to prepare micron-sized DNA flowers (DFs) with co-encapsulation and spatial regulation of bi-enzyme molecules, GOx and HRP. The cascade of GOx/HRP is regulated in the highly ordered water environment of DFs, leading to enhanced catalytic efficiency. The versatile application of bi-enzyme co-encapsulated DFs in sensitive and selective detection of cancerous exosomes and thrombin shows great potential in bioanalysis and disease diagnosis.

BIOSENSORS & BIOELECTRONICS (2021)

Article Nanoscience & Nanotechnology

Enzymatic Cascade Reactions Mediated by Highly Efficient Biomimetic Quasi Metal-Organic Frameworks

Huan Xia et al.

Summary: This study synthesized Imitative NH₂-MIL-101 with highly efficient peroxidase-like activity, which served as an excellent catalyst for oxidations of various peroxidase substrates and provided potential matrix for enzyme immobilization. The material also showed synergistic effect in enzymatic cascade reactions, enhancing the catalytic activity.

ACS APPLIED MATERIALS & INTERFACES (2021)

Article Chemistry, Multidisciplinary

Solid-Binding Peptide-Guided Spatially Directed Immobilization of Kinetically Matched Enzyme Cascades in Membrane Nanoreactors

Deniz T. Yucesoy et al.

Summary: This study demonstrates a modular cascade flow reactor using a solid-binding peptide-directed immobilization strategy on anodic aluminum oxide (AAO) monoliths for two-step biocatalytic production of L-lactate with concomitant regeneration of NADH. The strategy provides insight for controlling surface orientation, spatial localization, and loading of multiple enzymes on solid supports, enabling the construction of high-power cascadic devices.

ACS OMEGA (2021)

Article Multidisciplinary Sciences

Peptide sequence-driven direct electron transfer properties and binding behaviors of gold-binding peptide-fused glucose dehydrogenase on electrode

Hyeryeong Lee et al.

Summary: This study compared the effects of different gold-binding peptides fused to enzymes on electrocatalytic activity, bioactivity, and material-binding behaviors, demonstrating the vital role of GBP type in the design, construction, and optimization of enzyme-modified electrodes for efficient interfacial DET in DET-based enzyme-electrode systems.

ISCIENCE (2021)

Article Chemistry, Physical

Exploiting Bidirectional Electrocatalysis by a Nanoconfined Enzyme Cascade to Drive and Control Enantioselective Reactions

Lei Wan et al.

Summary: The ability to drive and observe rapid enzyme catalysis in both directions is achieved by immobilizing cascade components within electrode nanopores and coupling reactions to fast, quasi-reversible NADP(+)/ NADPH electrochemistry. By using two electrodes in sequence, each containing an enantioselective alcohol dehydrogenase for one halfcycle, efficient one-pot de-racemizers can be achieved.

ACS CATALYSIS (2021)

Article Chemistry, Physical

Reversible H2 oxidation and evolution by hydrogenase embedded in a redox polymer film

Steffen Hardt et al.

Summary: The research describes a redox-active film for bidirectional and reversible hydrogen conversion, which can be used for electrocatalytic energy conversion. By serving as both the anode material and a highly energy efficient cathode material in a biofuel cell, the film demonstrates high performance. The understanding of reversibility through a kinetic model has simplified the design principles of highly efficient and stable bioelectrocatalytic films.

NATURE CATALYSIS (2021)

Article Chemistry, Multidisciplinary

Bioelectrocatalytic Cofactor Regeneration Coupled to CO2 Fixation in a Redox-Active Hydrogel for Stereoselective C-C Bond Formation

Leonardo Castaneda-Losada et al.

Summary: This study demonstrates the electricity-driven regio- and stereoselective incorporation of CO2 into valuable products through bioelectrocatalysis, providing a promising approach for the conversion of CO2 into higher value-added molecules. The co-immobilization of enzymes for the regenerative conversion of CO2 into (2S)-ethylmalonyl-CoA with high faradaic efficiency and rate opens up new possibilities for more complex bioelectrocatalytic reactions in the future.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

Article Multidisciplinary Sciences

U1 snRNP regulates cancer cell migration and invasion in vitro

Jung-Min Oh et al.

NATURE COMMUNICATIONS (2020)

Review Chemistry, Multidisciplinary

Advancing photosystem II photoelectrochemistry for semi-artificial photosynthesis

Jenny Z. Zhang et al.

NATURE REVIEWS CHEMISTRY (2020)

Review Chemistry, Physical

Enzymatic electrosynthesis as an emerging electrochemical synthesis platform

Ranran Wu et al.

CURRENT OPINION IN ELECTROCHEMISTRY (2020)

Review Chemistry, Multidisciplinary

Advancing Techniques for Investigating the Enzyme-Electrode Interface

Nikolay Kornienko et al.

ACCOUNTS OF CHEMICAL RESEARCH (2019)

Review Chemistry, Physical

Multienzymatic Cascade Reactions via Enzyme Complex by Immobilization

Ee Taek Hwang et al.

ACS CATALYSIS (2019)

Article Chemistry, Multidisciplinary

Spatial Organization in Proteinaceous Membrane-Stabilized Coacervate Protocells

Junbo Li et al.

Article Biochemical Research Methods

X-ray structure of the direct electron transfer-type FAD glucose dehydrogenase catalytic subunit complexed with a hitchhiker protein

Hiromi Yoshida et al.

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY (2019)

Article Nanoscience & Nanotechnology

Constructing Submonolayer DNA Origami Scaffold on Gold Electrode for Wiring of Redox Enzymatic Cascade Pathways

Zhilei Ge et al.

ACS APPLIED MATERIALS & INTERFACES (2019)

Article Multidisciplinary Sciences

Occurrence of the potent mutagens 2-nitrobenzanthrone and 3-nitrobenzanthrone in fine airborne particles

Aldenor G. Santos et al.

SCIENTIFIC REPORTS (2019)

Article Chemistry, Multidisciplinary

Photoelectrochemistry of Photosystem II &ITin Vitro&IT vs&IT in Vivo&IT

Jenny Z. Zhang et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2018)

Article Chemistry, Physical

Photoelectrochemical reduction of carbon dioxide to methanol on p-type CuFe2O4 under visible light irradiation

Kaykobad Md Rezaul Karim et al.

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY (2018)

Editorial Material Multidisciplinary Sciences

CELL CYCLE Division enzyme regulates metabolism

Abigail S. Krall et al.

NATURE (2017)

Article Chemistry, Analytical

Bi-enzymatic biosensor for on-site, fast and reliable electrochemical detection of relevant D-amino acids in bacterial samples

Maria Moreno-Guzman et al.

SENSORS AND ACTUATORS B-CHEMICAL (2017)

Article Nanoscience & Nanotechnology

Mesoporous Metal-Organic Framework with Well-Defined Cruciate Flower-Like Morphology for Enzyme Immobilization

Jiandong Cui et al.

ACS APPLIED MATERIALS & INTERFACES (2017)

Article Chemistry, Multidisciplinary

Wiring of Photosystem II to Hydrogenase for Photoelectrochemical Water Splitting

Dirk Mersch et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2015)

Review Biotechnology & Applied Microbiology

Solid-binding peptides: smart tools for nanobiotechnology

Andrew Care et al.

TRENDS IN BIOTECHNOLOGY (2015)

Review Biochemical Research Methods

Cell-free metabolic engineering: Biomanufacturing beyond the cell

Quentin M. Dudley et al.

BIOTECHNOLOGY JOURNAL (2015)

Review Biotechnology & Applied Microbiology

Materials-Based Strategies for Multi-Enzyme Immobilization and Co-Localization: A Review

Feng Jia et al.

BIOTECHNOLOGY AND BIOENGINEERING (2014)

Article Nanoscience & Nanotechnology

Multi-enzyme complexes on DNA scaffolds capable of substrate channelling with an artificial swinging arm

Jinglin Fu et al.

NATURE NANOTECHNOLOGY (2014)

Article Multidisciplinary Sciences

A high-energy-density sugar biobattery based on a synthetic enzymatic pathway

Zhiguang Zhu et al.

NATURE COMMUNICATIONS (2014)

Article Chemistry, Physical

Design and Analysis of Enhanced Catalysis in Scaffolded Multienzyme Cascade Reactions

Jyun-Liang Lin et al.

ACS CATALYSIS (2014)

Article Chemistry, Multidisciplinary

Interenzyme Substrate Diffusion for an Enzyme Cascade Organized on Spatially Addressable DNA Nanostructures

Jinglin Fu et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2012)

Article Chemistry, Physical

Enzymatic Biofuel Cell for Oxidation of Glucose to CO2

Shuai Xu et al.

ACS CATALYSIS (2012)

Article Chemistry, Multidisciplinary

Biomimetic strategies for solar energy conversion: a technical perspective

Ardemis A. Boghossian et al.

ENERGY & ENVIRONMENTAL SCIENCE (2011)

Article Chemistry, Multidisciplinary

Engineering of Glucose Oxidase for Direct Electron Transfer via Site-Specific Gold Nanoparticle Conjugation

J. Todd Holland et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2011)

Article Chemistry, Multidisciplinary

Control of Biocatalytic Transformations by Programmed DNA Assemblies

Ronit Freeman et al.

CHEMISTRY-A EUROPEAN JOURNAL (2010)

Article Nanoscience & Nanotechnology

Enzyme cascades activated on topologically programmed DNA scaffolds

Ofer I. Wilner et al.

NATURE NANOTECHNOLOGY (2009)

Article Biochemical Research Methods

I-TASSER server for protein 3D structure prediction

Yang Zhang

BMC BIOINFORMATICS (2008)

Article Chemistry, Multidisciplinary

Effect of Molecular Conformations on the Adsorption Behavior of Gold-Binding Peptides

Marketa Hnilova et al.

LANGMUIR (2008)

Article Biochemical Research Methods

Protein production by auto-induction in high-density shaking cultures

FW Studier

PROTEIN EXPRESSION AND PURIFICATION (2005)

Article Biochemistry & Molecular Biology

Cloning and expression of the gene encoding catalytic subunit of thermostable glucose dehydrogenase from Burkholderia cepacia in Escherichia coli

K Inose et al.

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS (2003)

Article Chemistry, Analytical

Co-immobilization of lactate oxidase and lactate dehydrogenase on conducting polyaniline films

A Chaubey et al.

ANALYTICA CHIMICA ACTA (2000)