Double-Layer Hydrogel with Glucose-Activated Two-Stage ROS Regulating Properties for Programmed Diabetic Wound Healing

Slow healing of wounds induces great pain in diabetic patients. However, developing new approaches to promote diabetic wound healing is still one of the toughest challenges in the medical field. Here, we constructed a new double-layer hydrogel to effectively regulate reactive oxygen species (ROS) on the wound and promote diabetic wound healing. The inner layer contains glucose oxidase (Gox), ferrocene-modified quaternary ammonium chitosan (Fc-QCs), and poly(beta-cyclodextrin) (P beta-CD), which is used to generate hydroxyl radicals ((OH)-O-center dot) for antibacterial in the early stage of wound healing and collapses gradually. The outer layer is composed of gelatin and dopamine. In the later stage of wound healing, the outer layer contacts the skin, which is beneficial for ROS clearance on the wound. Antibacterial, ROS scavenging, and wound healing experiments have shown that the double-layer hydrogel possesses two-stage ROS regulating properties for programmed diabetic wound healing. In conclusion, it will be one of the most potential dressings for treating diabetic wounds in the future.

Automated summary

This study developed a new double-layer hydrogel for diabetic wound healing. The hydrogel demonstrated two-stage ROS regulation, antibacterial properties, and effective ROS clearance. This research provides a promising dressing option for treating diabetic wounds in the future.