Organic Phase-Soluble Nanomagnetically Cationic Phospholipid: Synthesis, Characterization, and In Vitro Transfection Activity

Thepresented work describes the synthesis and characterizationof a novel magnetic cationic phospholipid (MCP) system with a stabledopamine anchor as well as its transfection activity study. The synthesizedarchitectural system increases the biocompatibility of iron oxideand promises applications of magnetic nanoparticles in living cells.The MCP system is soluble in organic solvents and can be easily adaptedto prepare magnetic liposomes. We created complexes with liposomescontaining MCP and other functional cationic lipids and pDNA as genedelivery tools, which possessed the ability to enhance the efficiencyof transfection, particularly the process of interaction with cellsby inducing a magnetic field. The MCP is able to create iron oxidenanoparticles and has the potential for the materials to prepare thesystem for site-specific gene delivery with the application of anexternal magnetic field.

Automated summary

The presented work describes the synthesis and characterization of a novel magnetic cationic phospholipid (MCP) system with a stable dopamine anchor as well as its transfection activity study. The synthesized architectural system increases the biocompatibility of iron oxide and promises applications of magnetic nanoparticles in living cells. The MCP system is soluble in organic solvents and can be easily adapted to prepare magnetic liposomes. We created complexes with liposomes containing MCP and other functional cationic lipids and pDNA as gene delivery tools, which possessed the ability to enhance the efficiency of transfection, particularly the process of interaction with cells by inducing a magnetic field. The MCP is able to create iron oxide nanoparticles and has the potential for the materials to prepare the system for site-specific gene delivery with the application of an external magnetic field.