期刊
CHEMICAL COMMUNICATIONS
卷 51, 期 15, 页码 3266-3269出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4cc09765c
关键词
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资金
- NIH (IMAT) [R21-CA173279]
- University Cancer Research Fund (UNC)
- Lineberger Comprehensive Cancer Center (UNC)
- Carlson Foundation
- Triad Golfers Against Cancer
We report a novel strategy to enzymatically release affinity-selected cells, such as circulating tumor cells (CTCs), from surfaces with high efficiency (similar to 90%) while maintaining cell viability (>85%). The strategy utilizes single-stranded DNAs that link a capture antibody to the surfaces of a CTC selection device. The DNA linkers contain a uracil residue that can be cleaved.
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