A quick, cheap, and reliable protocol for immunofluorescence of pluripotent and differentiating mouse embryonic stem cells in 2D and 3D colonies

Summary: This study investigates the control of cell division in three-dimensional environments, finding that at the periphery of 3D colonies, embryonic stem cells exhibit high spindle mobility and asymmetric division. The research suggests that in 3D contexts, both the shape of the cell and its interactions with neighboring cells play key roles in regulating division orientation and symmetry.