Protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell-derived kidney organoids using Seahorse XF96

Article Cell & Tissue Engineering

SARS-CoV-2 infects the human kidney and drives fibrosis in kidney organoids

Jitske Jansen et al.

Summary: The study found that SARS-CoV-2 can directly infect kidney cells, leading to cell injury and fibrosis, causing kidney failure. Inhibitors can alleviate the infection.

CELL STEM CELL (2022)

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Article Biochemical Research Methods

Spatially resolved isotope tracing reveals tissue metabolic activity

Lin Wang et al.

Summary: Isotope imaging enables quantification of metabolic activity in mammalian tissues with spatial resolution, revealing metabolic heterogeneity in the kidney and brain and spatial gradients in metabolic processes.

NATURE METHODS (2022)

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Article Developmental Biology

Human pluripotent stem cell-derived kidney organoids for personalized congenital and idiopathic nephrotic syndrome modeling

Jitske Jansen et al.

Summary: Using organoid podocytes as a model, this study investigated idiopathic nephrotic syndrome and found that repaired organoids exhibited improved functionality, providing a valuable tool for disease modeling and therapy development.

DEVELOPMENT (2022)

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Review Medicine, General & Internal

Glucose Metabolism in Acute Kidney Injury and Kidney Repair

Lu Wen et al.

Summary: The kidneys play a crucial role in glucose homeostasis and abnormal glucose metabolism is associated with kidney diseases such as acute kidney injury. This review discusses the regulation of glucose homeostasis in kidneys and the impact of glucose metabolism on different types of AKI. Further research on glucose metabolism in kidney injury and repair could lead to the discovery of new therapeutic targets for kidney diseases.

FRONTIERS IN MEDICINE (2021)

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Article Biochemical Research Methods

Measurement of mitochondrial respiration in adherent cells by Seahorse XF96 Cell Mito Stress Test

Xia Gu et al.

Summary: Mitochondria play crucial roles in cellular energy metabolism, generating most of the intracellular ATP through respiration. The Cell Mito Stress Test is a common method for measuring key parameters of mitochondrial respiration, demonstrated here using the human cell line HK-2 as an example.

STAR PROTOCOLS (2021)

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Article Biochemical Research Methods

Protocol to profile the bioenergetics of organoids using Seahorse

Marlies C. Ludikhuize et al.

Summary: Addressing bioenergetics is crucial for evaluating the impact of metabolism on the regulation of biological processes and its alteration in disease. Organoids are in vitro self-organizing structures derived from healthy and diseased tissues that recapitulate tissue of origin accurately, but applying Seahorse XF analysis to organoid studies poses technical challenges.

STAR PROTOCOLS (2021)

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Article Multidisciplinary Sciences

Decoding myofibroblast origins in human kidney fibrosis

Christoph Kuppe et al.

Summary: Using single-cell RNA sequencing, researchers profiled the transcriptomes of healthy and fibrotic human kidneys, identifying distinct subpopulations of pericytes and fibroblasts as the main sources of scar-forming myofibroblasts during kidney fibrosis. This study shed light on the cellular origins and differentiation of myofibroblasts in human kidney fibrosis, identifying potential therapeutic targets such as NKD2.

NATURE (2021)

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Review Pediatrics