Protocol to evaluate the impact of murine MCT1- deficient CD8(+) T cells on adipogenesis

The infiltration of activated T cells, such as CD8(+) effector, in metabolic tissues plays a crucial role for the initiation and propagation of obesity-induced inflammation. Given the pivotal role of lactate transporter monocarboxylate transporter 1 (MCT1) in immune cell activation, we present a protocol for the isolation and activa-tion of CD8(+) T lymphocytes selectively lacking MCT1. We describe steps for the in-duction of adipocyte differentiation, CD8(+) T isolation and activation, and adipocyte-CD8(+) T cell co-culture. We then detail qPCR analysis on differentiated adipocytes. For complete details on the use and execution of this protocol, please refer to Macchi et al.(1)

Automated summary

The infiltration of activated T cells, such as CD8(+) effector, in metabolic tissues plays a crucial role in obesity-induced inflammation. The lactate transporter MCT1 is important for immune cell activation. This study provides a protocol for isolating and activating CD8(+) T lymphocytes lacking MCT1 and includes steps for adipocyte differentiation, CD8(+) T cell isolation and activation, and adipocyte-CD8(+) T cell co-culture. qPCR analysis on differentiated adipocytes is also detailed. For more information, refer to Macchi et al.(1).