4.1 Article

In cellulo and in vivo assays for compound testing cruzi

期刊

STAR PROTOCOLS
卷 4, 期 1, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.xpro.2023.102058

关键词

-

向作者/读者索取更多资源

We present a combined in cellulo and in vivo approach to identify compounds with high potential for inhibiting Trypanosoma cruzi. The in cellulo assays consist of two phases - one for excluding inactive or toxic compounds and the other for determining IC50, CC50, and selective index (SI). Compounds with high SI are further tested in in vivo infection models to evaluate their efficacy compared to benznidazole, a reference drug for Chagas disease treatment. For more details, refer to Marek et al. (2021).
Here, we describe a combined in cellulo and in vivo approach to identify com-pounds with higher potential for efficient inhibition of Trypanosoma cruzi. Phase I of in cellulo assays is designed to exclude inactive or toxic compounds, while phase II is designed for accurate IC50, CC50, and selective index (SI) determina-tion. Compounds showing high SI are tested using in vivo infection models in par-allel with benznidazole to assess their efficacy relative to a reference drug used for Chagas disease treatment. For complete details on the use and execution of this protocol, please refer to Marek et al. (2021).1

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.1
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据