4.6 Article

Microbial electrosynthetic nitrate reduction to ammonia by reversing the typical electron transfer pathway in Shewanella oneidensis

期刊

CELL REPORTS PHYSICAL SCIENCE
卷 4, 期 6, 页码 -

出版社

CELL PRESS
DOI: 10.1016/j.xcrp.2023.101433

关键词

-

向作者/读者索取更多资源

Ammonia production is achieved in a microbial electrosynthesis system using nitrate and an electrode as electron acceptors and donors. With the help of She-wanella oneidensis MR-1, the system achieves a high ammonia production rate of 24.3 mg h-1$mg protein -1 with 82.5% selectivity and 33.1% cathodic efficiency. The electrochemical analysis indicates the important roles of cytochromes c, flavins, and the flavin/c-cytochrome combination. The work proposes a progressive route for ammonia synthesis under mild conditions.
Ammonia production is a critical industrial process, and mild routes to recycle nitrates in wastewater could be a promising route to ammonia synthesis. In this study, ammonia production is demon-strated in a microbial electrosynthesis system with nitrate and an electrode as electron acceptors and donors, respectively. Based on the bidirectional extracellular electron transfer capability of She-wanella oneidensis MR-1, our microbial electrosynthetic system achieves a maximum ammonia production rate of 24.3 mg h-1$mg protein -1 with 82.5% selectivity and 33.1% cathodic efficiency and functions for several cycles over 30 days. Electrochemical analysis suggests that cytochromes c, flavins, and the flavin/c-cytochrome combination play a pivotal role. Charge transfer resistance weakens over the course of weeks, resulting in easier electron transfer. Paral-lel reaction monitoring proteomics suggest that reversing a typical Mtr pathwayplays a role, and a dissimilatory nitrate to ammonia pathway is used. This work proposes a progressive route to carry out ammonia synthesis under mild conditions.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据