4.2 Article

Preventing swarm detection in extracellular vesicle flow cytometry: a clinically applicable procedure

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Biochemical Research Methods

Automated fluorescence gating and size determination reduce variation in measured concentration of extracellular vesicles by flow cytometry

Angela Adriana Francisca Gankema et al.

Summary: This study aimed to investigate the impact of fluorophore brightness on the measurement of extracellular vesicle (EV) concentration. The researchers found that flow cytometry can detect and measure similar concentrations of EVs using different fluorophores and adding a size gate, contributing to the standardization of EV concentration measurements.

CYTOMETRY PART A (2022)

Article Immunology

Reliable measurements of extracellular vesicles by clinical flow cytometry

Martine Kuiper et al.

Summary: Extracellular vesicles (EVs) are cell-derived particles with potential to become biomarkers for disease diagnosis, prognosis, and monitoring. Flow cytometry is the only technique capable of rapidly detecting EVs, but challenges such as inconsistent units and sample preparation variations exist.

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2021)

Article Multidisciplinary Sciences

Standardized procedure to measure the size distribution of extracellular vesicles together with other particles in biofluids with microfluidic resistive pulse sensing

Michael Cimorelli et al.

Summary: In this study, the particle size distribution in biofluids was measured using microfluidic resistive pulse sensing (MRPS), revealing findings on optimal diluent, lower limit of detection, reproducibility, and agreement in concentration measurement. Operating guidelines were developed for reproducibly measuring the particle size distribution of extracellular vesicles (EVs) and other particles in biofluids with MRPS.

PLOS ONE (2021)

Article Medical Laboratory Technology

Comparison of Generic Fluorescent Markers for Detection of Extracellular Vesicles by Flow Cytometry

Leonie de Rond et al.

CLINICAL CHEMISTRY (2018)

Article Hematology

Flow cytometric analysis of extracellular vesicle subsets in plasma: impact of swarm by particles of non-interest

S. F. W. M. Libregts et al.

JOURNAL OF THROMBOSIS AND HAEMOSTASIS (2018)

Article Hematology

Standardization of extracellular vesicle measurements by flow cytometry through vesicle diameter approximation

E. van der Pol et al.

JOURNAL OF THROMBOSIS AND HAEMOSTASIS (2018)

Review Cardiac & Cardiovascular Systems

Extracellular Vesicles as Biomarkers in Cardiovascular Disease; Chances and Risks

Annemiek Dickhout et al.

FRONTIERS IN CARDIOVASCULAR MEDICINE (2018)

Article Hematology

Single vs. swarm detection of microparticles and exosomes by flow cytometry

E. Van der Pol et al.

JOURNAL OF THROMBOSIS AND HAEMOSTASIS (2012)

Article Biochemistry & Molecular Biology

Morphology and structure of lipoproteins revealed by an optimized negative-staining protocol of electron microscopy

Lei Zhang et al.

JOURNAL OF LIPID RESEARCH (2011)

Article Medical Laboratory Technology

Lipoprotein particle analysis by nuclear magnetic resonance spectroscopy

Elias J. Jeyarajah et al.

CLINICS IN LABORATORY MEDICINE (2006)