期刊
ACS APPLIED BIO MATERIALS
卷 6, 期 5, 页码 1774-1786出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsabm.3c00014
关键词
GelMA; gelatin; PCL; wound healing; wound dressing; nanofiber; mechanotransduction
This study presents a hydrogel-nanofiber composite wound dressing composed of GelMA and poly(caprolactone) (PCL)/gelatin nanofiber, which can systematically manage the skin regeneration process with enhanced mechanical property and micropatterned surface. The composite material promotes wound healing, stimulates reepithelialization and collagen deposition. It also regulates the morphology, proliferation, and collagen synthesis of fibroblasts, as well as the expression of alpha-SMA, TGF-beta, and collagen I and III during the wound healing process both in vitro and in vivo.
Wound dressings have been designed to provide the optimal environment to fibroblasts, keratinocytes, and macrophages to promote wound healing while inhibiting potential microbial infection. Gelatin methacrylate (GelMA) is a photopolymerizable hydrogel with a gelatin backbone that contains natural cell binding motifs such as arginine-glycine-aspartic acid (RGD) and MMP-sensitive degradation sites, making it an ideal material for wound dressing. However, GelMA alone is unable to stably protect the wound and regulate cellular activities due to its weak mechanical properties and nonmicropatterned surface, limiting its application as a wound dressing. Herein, we report the development of a hydrogel-nanofiber composite wound dressing utilizing GelMA and poly(caprolactone) (PCL)/gelatin nanofiber, which can systematically manage the skin regeneration process with an enhanced mechanical property and micropatterned surface. GelMA sandwiched between electrospun aligned and interlaced nanofibers that mimic epidermis and dermis layers, respectively, increased the stiffness of the resulting hydrogel composite with a comparable swelling rate as GelMA. Fabricated hydrogel composite was determined to be biocompatible and nontoxic. In addition to the beneficial effect of GelMA in accelerating wound healing, subsequent histological analysis revealed upregulated reepithelialization of granulation tissue and deposition of mature collagen. Hydrogel composite interacted with fibroblasts to regulate their morphology, proliferation, and collagen synthesis, as well as the expression of alpha-SMA, TGF-beta, and collagen I and III during the wound healing process both in vitro and in vivo. Taken together, we propose hydrogel/nanofiber composite as a wound dressing of the next generation that can induce skin tissue layer regeneration beyond the basic wound closure promotion of present dressings.
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