期刊
ACS APPLIED BIO MATERIALS
卷 6, 期 3, 页码 1252-1259出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsabm.3c00004
关键词
LNP; nanoparticles; tracing; DNA; hygiene
Effective cleaning and disinfection procedures are crucial for maintaining hygiene standards in health-care facilities. This study established a method using lipid nanoparticles (LNPs) encapsulating DNA to validate surface cleaning and disinfection procedures. The study showed that LNPs can distinguish between physical cleaning effects and chemical cleaning effects during the procedure. LNPs exhibited significant reductions in log10 values after treatment with ethanol and SDS solution, similar to reductions observed in common bacteria. LNPs offer advantages over existing tools for cleaning validation and enable separate detection of dilution and chemical disinfectant actions.
Effective cleaning and disinfection procedures are an integral part of good manufacturing practice and in maintaining hygiene standards in health-care facilities. In this study, a method to validate such cleaning and disinfection procedures of surfaces was established employing lipid nanoparticles (LNPs) encapsulating DNA. It was possible to determine and distinguish between the physical cleaning effect (dilution) and the chemical cleaning effect (disintegration) on the LNPs during the cleaning and disinfection procedure (wiping). After treatment with 70 v % ethanol as a disinfectant and SDS solution as a cleaning agent, LNPs showed log10 reductions of 4.5 and 4.0, respectively. These values are similar to the log10 reductions exhibited by common bacteria, such as Escherichia coli and Serratia marcescens. Therefore, LNPs pose as useful tools for cleaning validation with advantages over the already existing tools and enable a separate detection of dilution and chemical disinfectant action.
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