期刊
NON-CODING RNA
卷 9, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/ncrna9020017
关键词
microRNA; type 1 diabetes; biomarker; honeymoon; partial remission; childhood; immune regulation; epigenetics; islet autoimmunity; regulatory T cell
The partial remission phase of type 1 diabetes is characterized by endogenous insulin production and downregulated autoimmunity. This study identifies and validates a unique miRNA signature during this phase and demonstrates the immunomodulatory role of miR-30d-5p. Inhibition of miR-30d-5p results in increased regulatory T cells and changes in immune cell populations in vivo. This miRNA signature provides potential biomarkers and enhances understanding of the mechanisms driving the partial remission phase.
The partial remission (PR) phase of type 1 diabetes (T1D) is an underexplored period characterized by endogenous insulin production and downmodulated autoimmunity. To comprehend the mechanisms behind this transitory phase and develop precision medicine strategies, biomarker discovery and patient stratification are unmet needs. MicroRNAs (miRNAs) are small RNA molecules that negatively regulate gene expression and modulate several biological processes, functioning as biomarkers for many diseases. Here, we identify and validate a unique miRNA signature during PR in pediatric patients with T1D by employing small RNA sequencing and RT-qPCR. These miRNAs were mainly related to the immune system, metabolism, stress, and apoptosis pathways. The implication in autoimmunity of the most dysregulated miRNA, miR-30d-5p, was evaluated in vivo in the non-obese diabetic mouse. MiR-30d-5p inhibition resulted in increased regulatory T cell percentages in the pancreatic lymph nodes together with a higher expression of CD200. In the spleen, a decrease in PD-1(+) T lymphocytes and reduced PDCD1 expression were observed. Moreover, miR-30d-5p inhibition led to an increased islet leukocytic infiltrate and changes in both effector and memory T lymphocytes. In conclusion, the miRNA signature found during PR shows new putative biomarkers and highlights the immunomodulatory role of miR-30d-5p, elucidating the processes driving this phase.
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