期刊
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
卷 69, 期 -, 页码 1116-1124出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2016.08.016
关键词
Actin cytoskeleton; Cell morpholdgy; Ceramic; Osteoblast; Plasma chemical oxidation; Plasma polymer film; Titanium implant; Water contact angle; Surface charge
资金
- BMBF Germany Pilot Program Campus PlasmaMed [13N11183, 13N11188]
- Rostock University Medical Center (Research and Teaching) [889521]
Excellent osseointegration of permanent implants is crucial for the long lasting success of the implantation: To improve the osseointegrative potential, bio-inert titanium alloy surfaces (Ti6Al4V) are modified by plasma chemical oxidation (PCO (R)) of the titanium-oxide layer to a non-stoichiometric, amorphous calcium phosphate layer. The native titanium-oxide film measuring only a few nanometers is converted by PCO (R) to a thick porous calcium phosphate layer of about 10 mu m. In a second step the PCO surface is combined with a cell adhesive plasma-polymerized allylamine (PPAAm) nano film (5 and 50 nm). Independent of the PPAAm coating homogeneity, the human osteoblast-like MG-63 cells show a remarkable increase in cell Size and well-developed filopodia. Analyses of the actin cytoskeleton reveal that the cells mold to the pore Shape of the PPAAm-covered PCO, thereby establishing a strong attachment to the surface. Interestingly, we could demonstrate that even though our untreated PCO shows excellent hydrophilicity, this alone is not sufficient to facilitate fast cell spreading, but the positive surface charges mediated by PPAAm. This multilayet composite material guarantees enhanced interlocking of the cells with the porous surface. (C) 2016 Elsevier B.V. All rights reserved.
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