期刊
MARINE GENOMICS
卷 25, 期 -, 页码 95-102出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.margen.2015.12.001
关键词
Large yellow croaker; Liver transcriptome; Cold stress; Heat stress
资金
- National Natural Science Foundation of China [31172398]
- Zhejiang Provincial Natural Science Foundation of China [LY15C190005]
- Science and Technology Department of Ningbo Municipality [2015C110005]
- Zhejiang Provincial Top Key Discipline of Aquaculture in Ningbo University [XKZSC1410]
- K. C. Wong Magna Fund in Ningbo University
Large yellow croaker is an economically important marine fish in China. To understand the molecular mechanisms of this fish under temperature stress, liver transcriptomes of large yellow croakers undergoing heat and cold stress were investigated. The results showed that 130,246 contigs from cold stressed fish (CS), 109,203 contigs from control fish (NS) and 98,569 contigs from heat stressed fish (HS) were obtained from the liver transcriptomes with de novo analysis, respectively. And in total, 9467 (6113 up-regulated and 3364 down regulated), 5350 (2185 up-regulated and 3165 down-regulated), 10,622 (3146 up-regulated and 7477 down regulated) significantly differentially expressed genes were identified in CS-NS, HS-NS, and HS-CS, respectively. Pathway enrichment analysis showed that many pathways including those of energy metabolism and metabolic pathways were affected after temperature stress. Further qPCR analysis also confirmed that the expression levels of genes coding for key enzymes in metabolic pathways were dramatically changed. (C) 2015 Elsevier B.V. All rights reserved.
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