期刊
MARINE BIOTECHNOLOGY
卷 18, 期 2, 页码 168-175出版社
SPRINGER
DOI: 10.1007/s10126-016-9691-y
关键词
Sinonovacula constricta; Transcriptome; Development; Larval metamorphosis
资金
- National High Technology Research and Development Program of China (863 Program) [2012AA10A400-3]
- National Natural Science Foundation of China [31472278]
- Shanghai Universities Knowledge Service Platform [ZF1206]
The razor clam Sinonovacula constricta is an important commercial species. The deficiency of developmental transcriptomic data is becoming the bottleneck of further researches on the mechanisms underlying settlement and metamorphosis in early development. In this study, de novo transcriptome sequencing was performed for S. constricta at different early developmental stages by using Illumina HiSeq 2000 paired-end (PE) sequencing technology. A total of 112,209,077 PE clean reads were generated. De novo assembly generated 249,795 contigs with an average length of 585 bp. Gene annotation resulted in the identification of 22,870 unigene hits against the NCBI database. Eight unique sequences related to metamorphosis were identified and analyzed using real-time PCR. The razor clam reference transcriptome would provide useful information on early developmental and metamorphosis mechanisms and could be used in the genetic breeding of shellfish.
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