期刊
HELIYON
卷 9, 期 6, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.heliyon.2023.e16253
关键词
Probiotics; Enterococcus faecium; Oral cavity; WGS
This study aimed to isolate and investigate a bacterium from an Egyptian adult's healthy oral cavity, focusing on its probiotic properties, especially its antagonistic activity against oral pathogens. The bacterium NT04 was identified as Enterococcus faecium through 16S rRNA gene sequencing. Genomic analysis confirmed the presence of genes encoding diverse metabolic and probiotic properties in strain NT04. No pathogenicity islands or plasmid insertions were found. The genomic characteristics of strain NT04 support its potentiality as an anti-oral pathogen probiotic candidate.
Objective: This study aimed to isolate and investigate a bacterium from an Egyptian adult's healthy oral cavity, focusing on its probiotic properties, especially its antagonistic activity against oral pathogens. Methods: The isolated bacterium NT04 using 16S rRNA gene sequencing, was identified as Enterococcus faecium. In this study, the whole genome of Enterococcus faecium NT04 was sequenced and annotated by bioinformatics analysis tools. Results: Numerous genes encoding the production of diverse metabolic and probiotic properties, such as bacteriocin-like inhibitory substances (Enterocin A and B), cofactors, antioxidants, and vitamins, were confirmed by genomic analysis. There were no pathogenicity islands or plasmid insertions found. This strain is virulent for host colonization rather than invasion. Conclusion: Genomic characteristics of strain NT04 support its potentiality as an anti-oral pathogen probiotic candidate.
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