期刊
MACROMOLECULAR BIOSCIENCE
卷 17, 期 2, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201600236
关键词
cell infiltration; electrospun scaffolds; imaging; microstructure; X-ray nanotomography
资金
- EPSRC Impact Acceleration Fellowship [EP/M010538/1]
- EPSRC [EP/F007906/1, EP/M010619/1, EP/F028431/1, EP/I022562/1]
- Biotechnology and Biological Sciences Research Council (BBSRC, UK) [BB/H022597/1]
- BBSRC [BB/L026260/1, BB/H022597/1] Funding Source: UKRI
- EPSRC [EP/I022562/1, EP/M010619/1, EP/F028431/1, EP/M010538/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/H022597/1, BB/L026260/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/M010619/1, EP/I022562/1, EP/M010538/1, EP/F028431/1, EP/F007906/1] Funding Source: researchfish
Here, it is demonstrated that X-ray nanotomography with Zernike phase contrast can be used for 3D imaging of cells grown on electrospun polymer scaffolds. The scaffold fibers and cells are simultaneously imaged, enabling the influence of scaffold architecture on cell location and morphology to be studied. The high resolution enables subcellular details to be revealed. The X-ray imaging conditions were optimized to reduce scan times, making it feasible to scan multiple regions of interest in relatively large samples. An image processing procedure is presented which enables scaffold characteristics and cell location to be quantified. The procedure is demonstrated by comparing the ingrowth of cells after culture for 3 and 6 days.
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