期刊
FOOD CHEMISTRY-X
卷 18, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.fochx.2023.100682
关键词
Ganoderma lucidum; Polysaccharide; Structure; Antioxidant activity
In this study, Lactiplantibacillus plantarum ATCC14917 was used to ferment Ganoderma lucidum spore powder, and two polysaccharides were purified from unfermented (GLP) and fermented (FGLP) spore powder. The chemical structure and antioxidant activity of the polysaccharides were studied, and the effect of GLP and FGLP on the oxidative stress regulation pathway in HepG2 cells was explored.
In this study, Lactiplantibacillus plantarum ATCC14917 was used to ferment Ganoderma lucidum spore powder. Two polysaccharides were purified from unfermented (GLP) and fermented (FGLP) Ganoderma lucidum spore powder. The chemical structure and antioxidant activity of the polysaccharides were studied. Finally, the effect of GLP and FGLP on the oxidative stress regulation pathway in HepG2 cells was explored. The results showed that the main structural characteristics of Ganoderma lucidum polysaccharides remained unchanged during the fermentation. However, the average molecular weight (Mw) of Ganoderma lucidum polysaccharides decreased from 1.12 x 105 Da to 0.89 x 105 Da. Besides this, the contents of mannose, galactose, and glucuronic acid increased, while the contents of xylose and glucose were decreased. In addition, the content of uronic acid was raised, and the apparent structure was changed from smooth and hard to porous and loose. In antioxidant studies, intracellular ROS and MDA contents in the oxidative stress model were decreased, and T-AOC content was increased under GLP and FGLP intervention. In the investigation of the regulation pathway, Nrf-1 gene expression was up-regulated, and Keap1 gene expression was down-regulated under GLP and FGLP intervention. The antioxidant genes NQO1 and NO-1 expressions were increased to activate the activities of antioxidant enzymes CAT, SOD and GSH-PA to resist oxidative stress. Compared with GLP, FGLP has a stronger regulatory role in this pathway, thus showing more potent antioxidant activity. This experiment is beneficial to the further utilization of Ganoderma lucidum spore powder.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据