High Abundance of Candidatus Arthromitus in Intestinal Microbiota of Seriolella violacea (Palm Ruff) under Reared Conditions

Intestinal microbiota has been involved in several processes that benefit the host, such as digestion, nutrient metabolism, resistance to pathogens colonization and immune function. In this study, we investigated the diversity, composition and functional prediction of microbiota of reared Seriolella violacea (palm ruff) in the same cohort sampled at different times (7-, 8- and 9-month-old). Microbial community structure analyses, using 16S rRNA amplicon sequencing, revealed that the intestinal microbiota was dominated by the phyla Firmicutes, Proteobacteria, Fusobacteria and Tenericutes. At the genus level, Candidatus Arthromitus was the most abundant in all sampled timepoints, representing in average 78% of the bacterial community (ranging from 18 to 98%), corresponding to segmented filamentous bacteria, which are interesting because they have been associated with the maturation of immune responses in the gut and protecting the host from bacterial infections. The comparisons of the intestinal microbiota among the three groups showed differences in abundance of bacterial taxa and also in alpha diversity indexes (Shannon and Simpson), as well as beta diversity metrics (weighted and unweighted UniFrac). Potential functions of the intestinal microbiota of palm ruff were retrieved using Philipin and Tax4fun and these analyses revealed high levels of genes for sugar metabolism. To our knowledge, this study represents the first description of the intestinal microbiota of S. violacea.

Automated summary

This study investigated the diversity, composition and functional prediction of intestinal microbiota in reared Seriolella violacea. The dominant phyla were Firmicutes, Proteobacteria, Fusobacteria, and Tenericutes. The most abundant genus, Candidatus Arthromitus, had been associated with immune response maturation and protection against bacterial infections. Analysis also revealed high levels of genes for sugar metabolism in the intestinal microbiota of S. violacea.