期刊
MABS
卷 8, 期 6, 页码 1035-1044出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/19420862.2016.1190059
关键词
Antibody repertoire analysis; next-generation sequencing; ricin A chain; yeast surface display
资金
- Defense Threat Reduction Agency [HDTRA-1-13-1-0031]
Ricin is a toxin that could potentially be used as a bioweapon. We identified anti-ricin A chain antibodies by sequencing the antibody repertoire from immunized mice and by selecting high affinity antibodies using yeast surface display. These methods led to the isolation of multiple antibodies with high (sub-nanomolar) affinity. Interestingly, the antibodies identified by the 2 independent approaches are from the same clonal lineages, indicating for the first time that yeast surface display can identify native antibodies. The new antibodies represent well-characterized reagents for biodefense diagnostics and therapeutics development.
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