Characterizing the interaction conformation between T-cell receptors and epitopes with deep learning

Computational modelling of the interactions between T-cell receptors (TCRs) and epitopes is of great importance for immunotherapy and antigen discovery. However, current TCR-epitope interaction prediction tools are still in a relatively primitive stage and have limited capacity in deciphering the underlying binding mechanisms, for example, characterizing the pairwise residue interactions between TCRs and epitopes. Here we designed a new deep-learning-based framework for modelling TCR-epitope interactions, called TCR-Epitope Interaction Modelling at Residue Level (TEIM-Res), which took the sequences of TCRs and epitopes as input and predicted both pairwise residue distances and contact sites involved in the interactions. To tackle the current bottleneck of data deficiency, we applied a few-shot learning strategy by incorporating sequence-level binding information into residue-level interaction prediction. The validation experiments and analyses indicated its good prediction performance and the effectiveness of its design. We demonstrated three potential applications: revealing the subtle conformation changes of mutant TCR-epitope pairs, uncovering the key contacts based on epitope-specific TCR pools, and mining the intrinsic binding rules and patterns. In summary, our model can serve as a useful tool for comprehensively characterizing TCR-epitope interactions and understanding the molecular basis of binding mechanisms. Computational modelling of the interactions between T-cell receptors (TCRs) and epitopes is a crucial yet challenging scientific problem. Peng and colleagues develop a deep learning model to capture TCR-epitope binding patterns, providing useful insights for understanding TCR recognition.

Automated summary

Studying the interactions between T-cell receptors (TCRs) and epitopes is crucial for immunotherapy and antigen discovery. Researchers developed a new deep-learning-based model to predict TCR-epitope interactions, providing valuable insights into binding mechanisms.