Structural Properties of a Heteropolysaccharide Released from Isaria cicadae Miq. Solid-State Fermented Wheat Bran

The work aimed to improve the extraction efficiency of wheat bran polysaccharide by solid-state fermentation using the bioactive fungus Isaria cicadae Miq. and identify the structural properties of fermented wheat bran polysaccharide (IC-FWBP). The polysaccharide fraction of IC-FWBP was isolated with an extraction yield of 2.88% and an average molecular weight of 3.31 x 10(6) Da. The IC-FWBP was comprised of mannose, glucose, and galactose. The methylation, nuclear magnetic resonance (NMR), and Congo red analysis results suggested that IC-FWBP contained glycosidic linkages of T-beta-D-Glcf, 1 -> 2-alpha-D-Manp, 1 -> 5,6-beta-D-Galf, 1 -> 2,3,4-alpha-D-Galp and 1 -> 2,3,4-beta-D-Manp, with triple-helix conformations. The morphological observation showed that IC-FWBP was composed of rod-like and spherical particles. These investigations on the structural properties of IC-FWBP will be beneficial to further research on the functional properties of wheat bran polysaccharides.

Automated summary

The study aimed to improve the efficiency of wheat bran polysaccharide extraction through solid-state fermentation using the bioactive fungus Isaria cicadae Miq. The structural properties of the fermented wheat bran polysaccharide (IC-FWBP) were identified. The IC-FWBP consisted of mannose, glucose, and galactose, with glycosidic linkages and triple-helix conformations.