期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 72, 期 -, 页码 390-398出版社
ELSEVIER
DOI: 10.1016/j.lwt.2016.05.010
关键词
Glucan; Lactobacillus; Kefir; Biocompatibility; Food additive
资金
- Fundacao de Amparo a Pesquisa de Minas Gerais (FAPEMIG) [APQ-02353-10]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [559370/2010-5]
- Pro-Reitoria de Pesquisa/Universidade Federal de Minas Gerais (PRPq/UFMG) [ADRC-01/2013]
Lactic acid bacteria produce exopolysaccharides, which have broad application in the food industry, especially as thickeners, stabilizers, and gelling agents. Additionally, some biopolymers have been regarded as health promoters due to their role as prebiotics and their immunomodulatory properties. This study aimed to characterize three exopolysaccharides produced by novel Lactobacillus strains from Brazilian milk or sugar water kefir and to determine their biocompatibility. Lactobacillus kefiranofaciens 1P3 and Lactobacillus satsumensis 10P and 10P2 were grown in the presence of sucrose, and their exopolysaccharides were purified and structurally elucidated. Glucose was the only sugar found in the polysaccharide chains, so most of the glucose residues were linked by alpha-(1,6) glycosidic bonds (similar to 90%) and the estimated molecular weight was 800 kDa. According to the NIR spectra, alpha-glucan 1P3, 10P, and 10P2 were all different compounds. The in vitro cytotoxicity of the polymers was low or nonexistent, especially in nontumor cells. The polymers had no significant effects on the expression of proinflammatory and regulatory cytokines in the intestine of mice. In contrast, the intestinal IgA(+) B cells were significantly higher. Taken together, this study suggests that these exopolysaccharides might be useful as food additives due to their safety features to the host (C) 2016 Elsevier Ltd. All rights reserved.
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