期刊
ACS APPLIED NANO MATERIALS
卷 -, 期 -, 页码 -出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsanm.3c00174
关键词
self-assembly; nanotechnology; molecular dynamics; bacteria; biofilm; chirality; extracellular matrix
This study focuses on the characterization of PSM alpha 1 amyloid nanofibers in Staphylococcus aureus biofilms. Computational and experimental techniques were used to develop a model for designing compounds to control amyloid formation. The research found that the amyloid nanofibers have a helical structure formed by two protofilaments, with an average diameter of about 12 nm and a left-handed helicity with a periodicity of approximately 72 nm.
Amyloid nanofibers are abundant in microorganisms and are integral components of many biofilms, serving various purposes, from virulent to structural. Nonetheless, the precise characterization of bacterial amyloid nanofibers has been elusive, with incomplete and contradicting results. The present work focuses on the molecular details and characteristics of PSM alpha 1-derived functional amyloids present in Staphylococcus aureus biofilms, using a combination of computational and experimental techniques, to develop a model that can aid the design of compounds to control amyloid formation. Results from molecular dynamics simulations, guided and supported by spectroscopy and microscopy, show that PSM alpha 1 amyloid nanofibers present a helical structure formed by two protofilaments, have an average diameter of about 12 nm, and adopt a left-handed helicity with a periodicity of approximately 72 nm. The chirality of the self-assembled nanofibers, an intrinsic geometric property of its constituent peptides, is central to determining the fibers' lateral growth.
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