期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 72, 期 -, 页码 317-321出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.lwt.2016.05.009
关键词
Wine; Fermentation; High-throughput sequencing; Lactic acid bacteria; Yeast
资金
- Wildwine EU Project [315065]
- Beatriu de Pinos grant
- Ministry of Universities and Research of the Department of Economy and Knowledge of the Generalitat of Catalonia
- program COFUND Marie Curie Actions of the 7th Framework Program for R+D of EU
Understanding the diversity and evolution of microorganisms during wine fermentation is essential for controlling its production. Previous studies have been primarily based on culture-dependent methods but recent incorporation of culture-independent molecular methods is showing a quite different view of microbial composition and diversity during the wine making process. Herein we applied barcoded pyrosequencing technology to monitor bacterial and yeast dynamics during laboratory scale spontaneous wine fermentation from Grenache variety. Members of the lactic acid bacteria (LAB) and acetic acid bacteria (AAB) were the most abundant, representing the orders Lactobacillales and Rhodospirillales more than 70% of the bacterial population. Other bacterial genera, not previously detected at the end of fermentation, were present in low proportion and their possible role remains unknown. Within the yeast community, the genera Hanseniaspora and Candida were dominant during the initial and mid fermentation while the final fermentation was mainly dominated by Candida and Saccharomyces. This study contributes to the knowledge of the microbial dynamics across spontaneous wine fermentation and presents high-throughput sequencing as a useful tool to monitor and evaluate bacterial and yeast diversity and dynamics during wine fermentation. (C) 2016 Elsevier Ltd. All rights reserved.
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