Abscisic-Acid-Modulated Stomatal Conductance Governs High-Temperature Stress Tolerance in Rice Accessions

Rising air temperature is a major constraint for crop productivity under the current climate change scenario. Rice crops are known to be sensitive to high-temperature (HT) stress at anthesis and post-anthesis stages. Photosynthesis is an important metabolic process and is affected by HT stress. A pot study was planned to screen a set of seventy-three Indian rice accessions based upon changes in the rate of photosynthesis (Pn) and related gas exchange traits under HT, and to characterize the contrasting rice accessions for component traits of HT stress tolerance. All accessions were raised under ambient temperature (AT) until the booting stage and exposed to HT using controlled chambers at anthesis and post-anthesis. HT exposure led to a large reduction (up to 50%) in Pn, but stomatal conductance (g(s)) and the rate of transpiration (E) increased significantly across the rice accessions. Based on the photosynthetic response under HT, two contrasting rice accessions (IRGC 135883, tolerant, and IRGC 127222, sensitive) were selected and characterized for HT tolerance, along with an NL-44 check. Among them, Pn decreased marginally but g(s) and E showed significant increases under HT in the tolerant accession, while sensitive accession showed an up to 50% reduction in Pn and marginal increase in g(s) and E. No significant changes were recorded for chlorophyll fluorescence (Fv/Fm) in both the genotypes, but tissue temperature depression (TTD) was higher in IRGC 135883 accession under HT. Endogenous abscisic acid (ABA) content increased under HT in the flag leaf of both the accessions, and the highest increase was observed in the sensitive accession. Similarly, spikelet fertility and grain yield showed large reductions in sensitive rice accession under HT. A large increase in ABA concentration in the leaves of the sensitive rice accession might be affecting its g(s) and cooling capacity under an HT environment. Finally, the study concludes that tolerant rice accessions can be recommended as donors and exploited in future rice breeding programs for developing climate-resilient rice genotypes.

Automated summary

Rising air temperature is a major constraint for crop productivity. Rice crops are sensitive to high-temperature stress, which affects photosynthesis and gas exchange traits. A study screened seventy-three Indian rice accessions based on their photosynthetic response under high temperature and characterized two contrasting accessions for heat tolerance. The tolerant accession showed marginal decrease in photosynthesis but significant increase in stomatal conductance and transpiration, while the sensitive accession exhibited large reductions in photosynthesis and slight increases in stomatal conductance and transpiration. The study concludes that tolerant rice accessions can be used in breeding programs for developing climate-resilient rice genotypes.