4.5 Article

Airway Epithelial Cultures of Children with Esophageal Atresia as a Model to Study Respiratory Tract Disorders

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Critical Care Medicine

Low CC16 mRNA Expression Levels in Bronchial Epithelial Cells Are Associated with Asthma Severity

Xingnan Li et al.

Summary: This study investigated the association between CC16 mRNA expression levels in nonciliated bronchial epithelial cells (BECs) and asthma severity. The results showed that low CC16 mRNA expression levels were significantly associated with asthma susceptibility, severity, exacerbations, and T2 inflammation biomarkers. Additionally, CC16 mRNA expression levels were correlated with the expression levels of Th2, Th1, and inflammation genes. A combination of CC16 and IL-6 could identify different asthma endotypes.

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE (2023)

Article Microbiology

The Human Nose Organoid Respiratory Virus Model: an Ex Vivo Human Challenge Model To Study Respiratory Syncytial Virus (RSV) and Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Pathogenesis and Evaluate Therapeutics

Anubama Rajan et al.

Summary: Preclinical models that can accurately replicate human respiratory virus infections and evaluate the efficacy of immunotherapies are highly needed. In this study, researchers developed a noninvasive technique to generate human nose organoids (HNOs) as an alternative to invasive biopsy-derived organoids. They successfully cultured HNOs and infected them with respiratory syncytial virus (RSV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The infected HNOs exhibited key characteristics of RSV and SARS-CoV-2 infections, including viral shedding and immune responses. Additionally, the researchers demonstrated the feasibility of using the HNO-ALI model to test the efficacy of a therapeutic antibody for preventing RSV infection. This study provides a valuable alternative model for studying respiratory viruses and testing therapeutics.
Article Cell Biology

Multiomics Analysis of a DNAH5-Mutated PCD Organoid Model Revealed the Key Role of the TGF-β/BMP and Notch Pathways in Epithelial Differentiation and the Immune Response in DNAH5-Mutated Patients

Wenhao Yang et al.

Summary: This study established a child PCD airway organoid and revealed the association between abnormal ciliary function and decreased immune response caused by DNAH5 mutation through single-cell RNA sequencing and proteomic analyses.
Article Microbiology

Human Nasal Organoids Model SARS-CoV-2 Upper Respiratory Infection and Recapitulate the Differential Infectivity of Emerging Variants

Man Chun Chiu et al.

Summary: This article reports on an organoid culture system of the nasal epithelium, which is essential for understanding cell biology and virus-host interaction in the human upper respiratory tract. The system allows for efficient reconstruction and stable expansion of the human nasal epithelium, providing a convenient and robust tool for studying SARS-CoV-2 infection and assessing the infectivity of emerging variants.
Article Biology

Measuring cystic fibrosis drug responses in organoids derived from 2D differentiated nasal epithelia

Gimano D. Amatngalim et al.

Summary: This study introduces an alternative method of culturing nasal-brushing-derived airway organoids, enabling consistent detection of CFTR modulator responses in patients with cystic fibrosis.

LIFE SCIENCE ALLIANCE (2022)

Article Respiratory System

Imprinting of bronchial epithelial cells upon in vivo rhinovirus infection in people with asthma

Abilash Ravi et al.

Summary: This study reveals that bronchial epithelial cells in asthma patients have a defective translocation of the translational repressor TIAR, resulting in epithelial hyperresponsiveness. This hyperresponsiveness is further increased upon exposure to rhinovirus and applies to the bronchoconstrictor endothelin-1, potentially contributing to airway obstruction.

ERJ OPEN RESEARCH (2022)

Article Pediatrics

Rho/SMAD/mTOR triple inhibition enables long-term expansion of human neonatal tracheal aspirate-derived airway basal cell-like cells

Junjie Lu et al.

Summary: In this study, epithelial basal cell-like cell lines were derived from human neonatal tracheal aspirates using a combination of Rho/SMAD signaling inhibition and mTOR inhibition. These cells can be expanded long-term and differentiated into functional airway epithelium, providing a novel model for investigating perinatal lung development and diseases.

PEDIATRIC RESEARCH (2021)

Article Respiratory System

Higher throughput drug screening for rare respiratory diseases: readthrough therapy in primary ciliary dyskinesia

Dani Do Hyang Lee et al.

Summary: Development of therapeutic approaches for rare respiratory diseases is impeded by the lack of systems for screening differentiated respiratory epithelium from affected patients. A novel screening method has been developed which allows for extensive expansion of basal cells from PCD patients and differentiation into ciliated epithelium in miniaturised ALI cultures. This platform provides a higher throughput analysis of airway epithelia and suggests new avenues for drug evaluation in PCD caused by nonsense mutations.

EUROPEAN RESPIRATORY JOURNAL (2021)

Article Physiology

Disease modeling following organoid-based expansion of airway epithelial cells

Evelien Eenjes et al.

Summary: The study investigated the use of organoid expansion to establish air-liquid interface (ALI) cultures from clinical samples with low epithelial cell numbers. Cells from different sources and expanded in different ways showed variations in cellular composition, response to cigarette smoke exposure, and differentiation modulation. Organoid-based expansion proved to be a valid method for establishing ALI cultures from samples with low cell numbers.

AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY (2021)

Article Developmental Biology

Developmental basis of trachea-esophageal birth defects

Nicole A. Edwards et al.

Summary: TEDs, a spectrum of life-threatening congenital anomalies, have been poorly understood in terms of their developmental basis, but recent advances in genetics and organoids are revealing the molecular and cellular mechanisms behind them. By integrating developmental mechanisms from animals and organoids with human genetics and clinical data, we may gain insight into the genotype-phenotype basis of these heterogeneous birth defects, leading to enhanced diagnosis, improved treatment, and potentially new tissue replacement therapy.

DEVELOPMENTAL BIOLOGY (2021)

Article Pharmacology & Pharmacy

Kakkonto Inhibits Cytokine Production Induced by Rhinovirus Infection in Primary Cultures of Human Nasal Epithelial Cells

Natsumi Saito et al.

Summary: Kakkonto extract can reduce the production of specific pro-inflammatory cytokines in response to rhinovirus (RV) infection, suggesting its potential as an effective therapy to inhibit inflammation caused by RV and subsequent airway inflammation.

FRONTIERS IN PHARMACOLOGY (2021)

Article Pediatrics

Pulmonary function in children and adolescents after esophageal atresia repair

Felipe Donoso et al.

PEDIATRIC PULMONOLOGY (2020)

Article Biochemistry & Molecular Biology

Regenerative Metaplastic Clones in COPD Lung Drive Inflammation and Fibrosis

Wei Rao et al.

Article Immunology

Epithelial-macrophage-dendritic cell interactions impact alarmins expression in asthma and COPD

Magdalena Paplinska-Goryca et al.

CLINICAL IMMUNOLOGY (2020)

Article Multidisciplinary Sciences

Progenitor identification and SARS-CoV-2 infection in human distal lung organoids

Ameen A. Salahudeen et al.

NATURE (2020)

Article Medicine, General & Internal

A Revised Protocol for Culture of Airway Epithelial Cells as a Diagnostic Tool for Primary Ciliary Dyskinesia

Janice L. Coles et al.

JOURNAL OF CLINICAL MEDICINE (2020)

Article Biochemistry & Molecular Biology

Long-term expanding human airway organoids for disease modeling

Norman Sachs et al.

EMBO JOURNAL (2019)

Article Critical Care Medicine

Exhaustion of Airway Basal Progenitor Cells in Early and Established Chronic Obstructive Pulmonary Disease

Moumita Ghosh et al.

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE (2018)

Article Cell & Tissue Engineering

Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells

Hongmei Mou et al.

CELL STEM CELL (2016)

Article Biochemistry & Molecular Biology

Intrinsic Phenotypic Differences of Asthmatic Epithelium and Its Inflammatory Responses to Respiratory Syncytial Virus and Air Pollution

Tillie-Louise Hackett et al.

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY (2011)

Review Pediatrics

Long-term results of esophageal atresia: Helsinki experience and review of literature

Saara J. Sistonen et al.

PEDIATRIC SURGERY INTERNATIONAL (2011)

Article Respiratory System

Repaired oesophageal atresia: respiratory morbidity and pulmonary function in adults

S. Sistonen et al.

EUROPEAN RESPIRATORY JOURNAL (2010)

Article Biochemistry & Molecular Biology

Mutations in SOX2 cause anophthalmia-esophageal-genital (AEG) syndrome

KA Williamson et al.

HUMAN MOLECULAR GENETICS (2006)