期刊
LIFE-BASEL
卷 13, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/life13020490
关键词
organophosphate pesticides; carboxylesterase EST2; decontamination; phosphotriesterase SsoPox
In recent decades, the increased use of pesticides in agriculture has led to safety concerns for the environment and human health, necessitating the development of new detection and decontamination systems. A new qualitative test utilizing thermostable enzymes was discovered, allowing for the detection of pesticide residues on fruits and vegetables. By analyzing esterase activity, the presence of residual pesticides and their decontamination was successfully assessed.
Background: In recent decades, the use of pesticides in agriculture has increased at a fast pace, highlighting safety problems for the environment and human health, which in turn has made it necessary to develop new detection and decontamination systems for pesticides. Methods: A new qualitative test capable of detecting the presence of pesticides on fruits and vegetables by using thermostable enzymes was discovered, and the test was carried out on apples and aubergines. The contaminating pesticides were extracted from fruits with acetonitrile and analyzed with a biosensor system based on the thermostable esterase EST2 immobilized on a nitrocellulose filter. This enzyme is irreversibly inhibited mainly in the presence of organophosphates pesticides. Therefore, by observing esterase activity inhibition, we revealed the presence of residual pesticides on the fruits and vegetables. Results: By analyzing the rate of esterase activity inhibition, we predicted that residual pesticides are present on the surface of the fruits. When we cleaned the fruits by washing them in the presence of the phosphotriesterase SsoPox before the detection of the esterase activity on filters, we observed a full recovery of the activity for apples and 30% for aubergines, indicating that the enzymatic decontamination of organophosphates pesticides took place. Conclusions: The reported method permitted us to assess the pesticides present on the vegetables and their decontamination.
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