期刊
LIFE-BASEL
卷 13, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/life13030651
关键词
Ocimum forskolei; Ocimum forsskaolii; essential oil; GC-MS; HCT116 cancer cell line; apoptosis; cell cycle; bacteria; antimicrobial agent
This study analyzed the essential oil obtained from the flowering aerial parts of Forsskal's Basil Ocimum forskolei Benth, which grows in extreme environmental conditions in Mecca Region, Saudi Arabia. The main constituents of the essential oil were phenylpropanoids, monoterpene, and sesquiterpenes. The essential oil showed inhibitory effects on colon cancer cells and demonstrated antimicrobial activity against various bacteria.
Many species belonging to the genus Ocimum are used for aromatic, medicinal, and cosmetic purposes. The essential oil (OFEO) obtained by hydrodistillation of the flowering aerial parts of Forsskal's Basil Ocimum forskolei Benth growing in extreme environmental conditions in Mecca Region, Saudi Arabia was analyzed by GC-MS. The main constituents were phenylpropanoids (methyl eugenol 55.65% and eugenol 11.66%), monoterpene (linalool 9.75%), and sesquiterpenes (germacrene D 3.72% and beta-caryophyllene 2.57%). The OFEO was tested against MCF7, HT29, and HCT116 cancer cells and compared with normal fibroblast cells (MRC5). The MTT assay showed that HCT116 was more sensitive to OFEO (IC50 5.34 mu g/mL), which reduced the number of HCT116 colonies at 6 mu g/mL, while causing complete colony death at 12 and 24 mu g/mL. Western Blotting and qRT-PCR were used to evaluate the level change of different proteins with respect to GAPDH. OFEO upregulated the apoptotic protein (caspase 3), and downregulated the cell proliferation proteins (AKT and pAKT), cell cycle arrest (PCNA, Cyclin D1), and the anti-apoptotic Bcl2 proteins. OFEO was also tested against reference strains of Gram-negative and Gram-positive bacteria including Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, and Staphylococcus aureus by using the well-diffusion and assessing their MICs, which ranged from 250 to 500 mu g/mL.
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