Rapid Matching Antibodies Pair and Fast Detecting Melioidosis with Fluorescent Immunochromatographic Test Strips

The high infectivity, difficulty to diagnose, and high drug resistance of melioidosis limited the timeliness of treatment. Lateral flow assay (LFA) was operated in this research to provide an instant diagnosis method, and a fast antibody rapid matching test strategy based on LFA was developed to select the most sensitive and specific pair of antibodies. Compared to the traditional ELISA method, the new matching strategy limits the pairing time to 3 h without any complex instruments. The rapid pairing test strategy is a universal strategy that is suitable for various sandwich immune antigen pairings. To fasten the test of the test strips, dry fluorescence immunoassay analyzer (DFIA) was designed and applied. The equipment also simplifies the process of data acquisition. Finally, the concentration gradient test was operated, and the detection lines and limits were presented.

Automated summary

This research utilized lateral flow assay (LFA) as an instant diagnosis method for melioidosis and developed a fast antibody rapid matching test strategy based on LFA to select the most sensitive and specific pair of antibodies. The new matching strategy reduced pairing time to 3 hours without requiring complex instruments compared to the traditional ELISA method. A dry fluorescence immunoassay analyzer (DFIA) was designed and applied to expedite the testing process of test strips, as well as simplify data acquisition. The concentration gradient test was conducted to analyze the detection lines and limits.