4.5 Article

Bioexposure assays to assess uptake and survival of viruses in mealworm (Tenebrio molitor) and black soldier fly (Hermetia illucens) larvae

期刊

JOURNAL OF INSECTS AS FOOD AND FEED
卷 9, 期 9, 页码 1165-1175

出版社

WAGENINGEN ACADEMIC PUBLISHERS
DOI: 10.3920/JIFF2022.0167

关键词

coronavirus; feed safety; insect rearing; virus survival; virus transmission

向作者/读者索取更多资源

This study developed virus bioexposure assays to examine the uptake and survival of porcine respiratory coronavirus in mealworm and black soldier fly larvae. The results showed that PRCV RNA could be detected in both larvae for up to 3 days after exposure.
Insects represent a sustainable source of food and feed. In order to increase the sustainability of insect production, new feed substrates should be explored including waste materials that contain animal by- products. However, the use of waste products does come with potential risks for transmission of pathogens. Experimental exposure studies of insects with selected pathogens can provide knowledge of some of these risks for future quantitative risk assessments. In this study, we have developed virus bioexposure assays to examine the uptake and survival of two different strains of porcine respiratory coronavirus (PRCV) in the larvae of two key insect species that are grown for the production of food and feed, namely the mealworm, Tenebrio molitor, and the black soldier fly, Hermetia illucens. T. molitor larvae were exposed to PRCV via oral uptake of virus suspensions while H. illucens larvae were exposed to PRCV via spiking of their feed. Using RT-qPCR assays, the amount of viral RNA extracted from individual larvae immediately after exposure varied from similar to 10(4.6) to 10(7.1) genome copies per insect. The PRCV RNA was detectable in the larvae of both T. molitor and H. illucens for up to 3 days post exposure but no longer. The assays applied to T. molitor and H. illucens for exposure to PRCV should be applicable for the exposure of further insect larvae to other viruses, and potentially also to other types of pathogens.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据