期刊
BIOSENSORS-BASEL
卷 13, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/bios13030312
关键词
hydrogels; surface micropattern; probe immobilization; photoclick reaction; diffraction; label-free
The present research aimed to develop a label-free biosensing platform using a biofunctionalized hydrogel with a surface diffractive micropattern. The biosensors were fabricated by holographic recording of polyethylene terephthalate (PET) surface micro-structures and their transfer into a hydrogel material. Two different immobilization approaches, during or after hydrogel synthesis, were demonstrated with the latter showing better loading capacity of the bioreceptor groups. The label-free biosensor showed selective response with a limit of detection of 2.47 μM for the complementary DNA strand, making it a cheap and easy-to-fabricate tool for medical diagnosis.
The present research is focused on the development of a biofunctionalized hydrogel with a surface diffractive micropattern as a label-free biosensing platform. The biosensors described in this paper were fabricated with a holographic recording of polyethylene terephthalate (PET) surface micro-structures, which were then transferred into a hydrogel material. Acrylamide-based hydrogels were obtained with free radical polymerization, and propargyl acrylate was added as a comonomer, which allowed for covalent immobilization of thiolated oligonucleotide probes into the hydrogel network, via thiol-yne photoclick chemistry. The comonomer was shown to significantly contribute to the immobilization of the probes based on fluorescence imaging. Two different immobilization approaches were demonstrated: during or after hydrogel synthesis. The second approach showed better loading capacity of the bioreceptor groups. Diffraction efficiency measurements of hydrogel gratings at 532 nm showed a selective response reaching a limit of detection in the complementary DNA strand of 2.47 mu M. The label-free biosensor as designed could significantly contribute to direct and accurate analysis in medical diagnosis as it is cheap, easy to fabricate, and works without the need for further reagents.
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