期刊
TOXICS
卷 11, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/toxics11030199
关键词
silver nanoparticles; Ag NP; intestine; toxicity; simulated gastrointestinal fluids; in vitro digestion; in vitro; HCT116; AOP-Wiki
The toxicity of silver nanoparticles (Ag NPs) in human intestinal cells was investigated in this study. The NPs were either uncoated or coated with polyvinylpyrrolidone (Ag PVP) or hydroxyethylcellulose (Ag HEC) and digested in simulated gastrointestinal fluids. Physicochemical transformations during digestion were analyzed before assessing toxicity. The evaluation strategy was based on adverse outcome pathways (AOPs) and included assessing cytotoxicity, oxidative stress, genotoxicity, cell cycle perturbation, and apoptosis. Ag NPs exhibited concentration-dependent toxicity, causing cell death, oxidative stress, DNA damage, and cell cycle disruption. In vitro digestion did not significantly affect their toxicity, except for genotoxicity. These findings suggest potential toxicity of ingested Ag NPs, which varied with coating but was not altered by digestion.
The widespread use of silver nanoparticles (Ag NPs) in food and consumer products suggests the relevance of human oral exposure to these nanomaterials (NMs) and raises the possibility of adverse effects in the gastrointestinal tract. The aim of this study was to investigate the toxicity of Ag NPs in a human intestinal cell line, either uncoated or coated with polyvinylpyrrolidone (Ag PVP) or hydroxyethylcellulose (Ag HEC) and digested in simulated gastrointestinal fluids. Physicochemical transformations of Ag NPs during the different stages of in vitro digestion were identified prior to toxicity assessment. The strategy for evaluating toxicity was constructed on the basis of adverse outcome pathways (AOPs) showing Ag NPs as stressors. It consisted of assessing Ag NP cytotoxicity, oxidative stress, genotoxicity, perturbation of the cell cycle and apoptosis. Ag NPs caused a concentration-dependent loss of cell viability and increased the intracellular level of reactive oxygen species as well as DNA damage and perturbation of the cell cycle. In vitro digestion of Ag NPs did not significantly modulate their toxicological impact, except for their genotoxicity. Taken together, these results indicate the potential toxicity of ingested Ag NPs, which varied depending on their coating but did not differ from that of non-digested NPs.
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