Guamara and Cocuixtle: Source of Proteases for the Transformation of Shrimp By-Products into Hydrolysates with Potential Application

Guamara and cocuixtle fruits are underutilized and the need arises to use them because they can favor the production of hydrolyzates, in addition to the fact that white shrimp is the crustacean that is produced in the greatest quantity in Mexico, of which approximately 50% of the total weight of shrimp is wasted. The objective of this research was to determine the optimum conditions for obtaining protein hydrolyzates from cooked shrimp waste, since it has been mentioned that cooking the shells improves their digestibility. This study is of vital importance for the utilization of resources, in addition to opening the possibility of using a new drug with antioxidant capacity or, failing that, to be used as a food additive to produce functional foods. Since the fruits of Bromelia pinguin and Bromelia karatas are rich in proteases, the aim of this research was to optimize the hydrolysis process of cooked white shrimp by-products due to the effect of these proteases. A robust Taguchi L-16' 0 design was used to optimize the hydrolysis process. Similarly, the amino acid profile by GC-MS and antioxidant capacity (ABTS and FRAP) were determined. The optimal conditions for hydrolysis of cooked shrimp by-products were pH 8.0, 30 degrees C, 0.5 h, 1 g of substrate and 100 mu g/mL of B. karatas, pH 7.5, 40 degrees C, 0.5 h, 0.5 g substrate and 100 mu g/mL enzyme extract from B. pinguin and pH 7.0, 37 degrees C, 1 h, 1.5 g substrate and 100 mu g/mL enzyme bromelain. The optimized hydrolyzates of B. karatas B. pinguin and bromelain had 8 essential amino acids in their composition. The evaluation of the antioxidant capacity of the hydrolyzates under optimal conditions showed more than 80% inhibition of in ABTS radical, B. karatas hydrolyzates had better higher ferric ion reduction capacity with 10.09 +/- 0.02 mM TE/mL. Finally, the use of proteolytic extracts from B. pinguin and B. karatas to optimize hydrolysis process allowed obtaining hydrolyzates of cooked shrimp by-products with potential antioxidant capacity.

Automated summary

The goal of this research was to determine the optimal conditions for obtaining protein hydrolyzates from cooked shrimp waste by using the proteases in Guamara and cocuixtle fruits. This study is of great importance for resource utilization and opens the possibility of using the hydrolyzates as a new drug with antioxidant capacity or as a food additive to produce functional foods.