4.7 Article

Blood-feeding adaptations and virome assessment of the poultry red mite Dermanyssus gallinae guided by RNA-seq

期刊

COMMUNICATIONS BIOLOGY
卷 6, 期 1, 页码 -

出版社

NATURE PORTFOLIO
DOI: 10.1038/s42003-023-04907-x

关键词

-

向作者/读者索取更多资源

Dermanyssus gallinae is a blood-feeding mite that parasitises wild birds and farmed poultry. Through transcriptome analysis, we identified midgut-enriched transcripts and upregulated cysteine protease transcripts in response to blood meals. We also discovered three distinct vitellogenin transcripts related to reproductive capacity and investigated genes associated with blood consumption, iron storage, and the RNA-virome of D. gallinae.
Dermanyssus gallinae is a blood-feeding mite that parasitises wild birds and farmed poultry. Its remarkably swift processing of blood, together with the capacity to blood-feed during most developmental stages, makes this mite a highly debilitating pest. To identify specific adaptations to digestion of a haemoglobin-rich diet, we constructed and compared transcriptomes from starved and blood-fed stages of the parasite and identified midgut-enriched transcripts. We noted that midgut transcripts encoding cysteine proteases were upregulated with a blood meal. Mapping the full proteolytic apparatus, we noted a reduction in the suite of cysteine proteases, missing homologues for Cathepsin B and C. We have further identified and phylogenetically analysed three distinct transcripts encoding vitellogenins that facilitate the reproductive capacity of the mites. We also fully mapped transcripts for haem biosynthesis and the ferritin-based system of iron storage and inter-tissue trafficking. Additionally, we identified transcripts encoding proteins implicated in immune signalling (Toll and IMD pathways) and activity (defensins and thioester-containing proteins), RNAi, and ion channelling (with targets for commercial acaricides such as Fluralaner, Fipronil, and Ivermectin). Viral sequences were filtered from the Illumina reads and we described, in part, the RNA-virome of D. gallinae with identification of a novel virus, Red mite quaranjavirus 1. Genes associated with the consumption of blood and iron storage are identified in the poultry red mite, along with presentation of a partial virome.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据