Comprehensive analysis of bZIP transcription factors in passion fruit

The bZIP transcription factors are well-known transcriptional regulators that are essential for regulating resistance to biotic and abiotic stresses in plants. In this study, a total of 56 putative bZIP members were identified in passion fruit (Passiflora edulis). An integrative analysis was performed using bioinformatics. Transcriptome analysis revealed that most PebZIPs respond to drought, salt, cold and heat stress. By combining the transcriptome results of two different resistant genotypes, four representative members were finally selected for differential expression validation in different tissues and cultivars. Furthermore, transcriptome and metabolome association analysis revealed consistent expression trends of PeZIP20 and PeZIP21, with only one difference at 63aa, with different metabolites including flavonoids, lipids and amino acids. This work will contribute to further studies of the functions of bZIPs and their resistance properties, as well as to the development of novel germplasm.

Automated summary

In this study, 56 putative bZIP members were identified in passion fruit and analyzed using bioinformatics. The transcriptome analysis revealed their response to various stresses, and the expression of four representative members was validated in different tissues and cultivars. The association analysis of transcriptome and metabolome further identified the consistent expression trends and differences in metabolites between PeZIP20 and PeZIP21. This research provides important insights into the functions and resistance properties of bZIPs, as well as the development of novel germplasm.