期刊
JOURNAL OF FUNGI
卷 9, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/jof9050535
关键词
Sporothrix; cerebrospinal fluid; molecular diagnosis; immunological diagnosis; qPCR; ELISA
Sporotrichosis, a subcutaneous mycosis, can lead to several complications such as meningeal forms in immunocompromised individuals. However, the diagnosis of meningeal sporotrichosis is time-consuming due to the limitations of culture and the low fungal burden in cerebrospinal fluid (CSF) samples. This study evaluated five non-culture-based methods for detecting Sporothrix spp. in CSF and found that molecular and immunological tests had substantial sensitivity and specificity for indirect detection. Implementation of these methods in clinical practice could improve early detection, treatment optimization, and prognosis of affected individuals.
Sporotrichosis is the main subcutaneous mycosis worldwide. Several complications, including meningeal forms, can be observed in immunocompromised individuals. The sporotrichosis diagnosis is time-consuming due to the culture's limitations. The low fungal burden in cerebrospinal fluid (CSF) samples is another important drawback in the diagnosis of meningeal sporotrichosis. Molecular and immunological tests can improve the detection of Sporothrix spp. in clinical specimens. Therefore, the following five non-culture-based methods were evaluated for the detection of Sporothrix spp. in 30 CSF samples: (i) species-specific polymerase chain reaction (PCR); (ii) nested PCR; (iii) quantitative PCR; (iv) enzyme-linked immunosorbent assay (ELISA) for IgG detection; and (v) ELISA for IgM detection. The species-specific PCR was unsuccessful in the diagnosis of the meningeal sporotrichosis. The other four methods presented substantial levels of sensitivity (78.6% to 92.9%) and specificity (75% to 100%) for the indirect detection of Sporothrix spp. Both DNA-based methods presented similar accuracy (84.6%). Both ELISA methods were concomitantly positive only for patients with sporotrichosis and clinical signs of meningitis. We suggest that these methods should be implemented in clinical practice to detect Sporothrix spp. in CSF early, which may optimize treatment, augment the chances of a cure, and improve the prognosis of affected individuals.
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