Genome Sequencing and Analysis Reveal Potential High-Valued Metabolites Synthesized by Lasiodiplodia iranensis DWH-2

Lasiodiplodia sp. is a typical opportunistic plant pathogen, which can also be classified as an endophytic fungus. In this study, the genome of a jasmonic-acid-producing Lasiodiplodia iranensis DWH-2 was sequenced and analyzed to understand its application value. The results showed that the L. iranensis DWH-2 genome was 43.01 Mb in size with a GC content of 54.82%. A total of 11,224 coding genes were predicted, among which 4776 genes were annotated based on Gene Ontology. Furthermore, the core genes involved in the pathogenicity of the genus Lasiodiplodia were determined for the first time based on pathogen-host interactions. Eight Carbohydrate-Active enzymes (CAZymes) genes related to 1,3-beta-glucan synthesis were annotated based on the CAZy database and three relatively complete known biosynthetic gene clusters were identified based on the Antibiotics and Secondary Metabolites Analysis Shell database, which were associated with the synthesis of 1,3,6,8-tetrahydroxynaphthalene, dimethylcoprogen, and (R)-melanin. Moreover, eight genes associated with jasmonic acid synthesis were detected in pathways related to lipid metabolism. These findings fill the gap in the genomic data of high jasmonate-producing strains.

Automated summary

In this study, the genome of a high jasmonate-producing strain, Lasiodiplodia iranensis DWH-2, was sequenced and analyzed. The genome was found to be 43.01 Mb in size with a GC content of 54.82%. Core genes involved in the pathogenicity of Lasiodiplodia genus, carbohydrate-active enzymes genes, and known biosynthetic gene clusters were identified. These findings provide important genomic data for understanding the application value of L. iranensis DWH-2.