期刊
BIOMEDICINES
卷 11, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/biomedicines11030660
关键词
DAO enzyme; AOC1; fibromyalgia; variants; SNPs; genetic deficiency; histamine intolerance
The study aimed to determine the prevalence of DAO deficiency in Spanish women with fibromyalgia and compare it with the European population. Results showed a DAO deficiency prevalence of 74.5% based on four AOC1 gene variants. The allele and genotypic frequencies did not differ from the European population, suggesting that AOC1 gene variants associated with DAO deficiency could be a potential biomarker for fibromyalgia.
Diamine oxidase (DAO) is an enzyme that metabolizes intestinal histamine. Single nucleotide polymorphisms (SNPs) of the Amine Oxidase Copper Containing 1 (AOC1) gene can lead to low enzymatic activity or functionality in histamine metabolism. This study aimed to determine the prevalence of DAO deficiency for four variants of the AOC1 gene, p.Thr16Met (rs10156191), p.Ser332Phe (rs1049742), p.His664Asp (rs1049793), and c.691G > T (rs2052129), in 98 Spanish women with fibromyalgia between the ages of 33 and 60 years, and compare the distribution of allelic and genotypic frequencies with those of European population samples in Hardy-Weinberg equilibrium extracted from the Allele Frequency Aggregator (ALFA) database. The patients' DNA was extracted, and analyzed using SNPE Multiplex (Single Nucleotide Primer Extension). The prevalence of genetic DAO deficiency was 74.5% based on the four variants of the AOC1 gene. SNP deficits were found at frequencies of 53.1% for p.Thr16Met, 49% for c.691G > T, 48% for p.His664Asp, and 19.4% for p.Ser332Phe. The allele and genotypic frequencies of the women with fibromyalgia did not differ from the European population. Variants of the AOC1 gene that are associated with genetic DAO deficiency could serve as a disruptive biomarker in patients with fibromyalgia. This study was registered in ClinicalTrials.gov Identifier: NCT05389761.
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