期刊
BIOMARKER RESEARCH
卷 11, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s40364-023-00508-2
关键词
ICOS; ICOS DNA methylation; Epigenetics; Immune checkpoint blockade; Melanoma; Predictive biomarker
In this study, the epigenetic regulation of ICOS in melanoma by DNA methylation was investigated. High ICOS mRNA expression was associated with enriched immune cell infiltration and favorable overall survival in non-ICB-treated patients. ICOS hypomethylation correlated with poor overall survival in non-ICB patients but predicted higher response and prolonged survival in ICB-treated patients. The study also revealed cytoplasmic and sporadic nuclear tumor cell-intrinsic ICOS protein expression, which could be induced by pharmacological demethylation with decitabine.
BackgroundInducible T cell costimulator ICOS is an emerging target in immuno-oncology. The aim of this study was to investigate the epigenetic regulation of ICOS in melanoma by DNA methylation.MethodsWe comprehensively investigate ICOS DNA methylation of specific CpG sites and expression pattern within the melanoma microenvironment with regard to immune correlates, differentiation, clinical outcomes, and immune checkpoint blockade (ICB) response.ResultsOur study revealed a sequence-contextual CpG methylation pattern consistent with an epigenetically regulated gene. We found a cell type-specific methylation pattern and locus-specific correlations and associations of CpG methylation with ICOS mRNA expression, immune infiltration, melanoma differentiation, prognosis, and response to ICB. High ICOS mRNA expression was identified as a surrogate for enriched immune cell infiltration and was associated with favorable overall survival (OS) in non-ICB-treated patients and predicted response and a prolonged progression-free survival (PFS) following ICB therapy initiation. ICOS hypomethylation, however, significantly correlated with poor OS in non-ICB patients but predicted higher response and prolonged PFS and OS in ICB-treated patients. Moreover, we observed cytoplasmic and sporadically nuclear tumor cell-intrinsic ICOS protein expression. Tumor cell-intrinsic ICOS protein and mRNA expression was inducible by pharmacological demethylation with decitabine.ConclusionOur study identified ICOS DNA methylation and mRNA expression as promising prognostic and predictive biomarkers for immunotherapy in melanoma and points towards a hitherto undescribed role of ICOS in tumor cells.
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