期刊
CURRENT OPINION IN ELECTROCHEMISTRY
卷 40, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.coelec.2023.101333
关键词
DNA; DNA-protein interaction; Biosensor; Electrochemical detection
Proteins in the cytoplasm bind selectively and sensitively to DNA, leading to structural changes in both biomolecules. Detection of these changes requires sophisticated analytical methods, with electrochemical detection being particularly suitable. This has laid the foundations for the development of biosensors.
Proteins present in the dense environment of the cytoplasm bind selectively with surprising sensitivity to DNA. Binding involves direct and indirect DNA recognition by a protein and is accompanied by a sequence of structural changes in both biomolecules. At the molecular level, they are expressed by a relocation of the base pairs, hydration, and hydrogen bond changes at the sugar-phosphate and base-stacking regions affecting the DNA biopolymer's curvature and conformation. Sophisticated analytical methods have to be employed to directly detect these subtle changes. Electrochemical detection proved to be very suitable for sensing structural changes in DNA, thus laying the foundations for the development of biosensors.
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