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Fluorescence for spatially and temporally resolved electrochemical imaging

CURRENT OPINION IN ELECTROCHEMISTRY (2023)

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Article Chemistry, Multidisciplinary

Wireless Electronic Light Emission: An Introduction to Bipolar Electrochemistry

Anne de Poulpiquet et al.

Summary: Electrochemistry is an important topic taught in undergraduate chemistry programs, but students often find it difficult due to the combination of various physical concepts involved. Bipolar electrochemistry is of special interest for teaching purposes, as it has a simple setup and allows for the tuning of electrochemical reactions through manipulating the electric field. Basic experiments using a commercially available light emitting diode (LED) as a bipolar electrode are introduced here, illustrating the key parameters driving electrochemical reactions and providing an eye-catching introduction to general electrochemistry.

JOURNAL OF CHEMICAL EDUCATION (2023)

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Article Chemistry, Multidisciplinary

Electrofluorochromic imaging analysis of dopamine release from living PC12 cells with bipolar nanoelectrodes array

Zhaoyan Tian et al.

Summary: The coupling of bipolar electrode (BPE) arrays and electrofluorochromic (EFC) imaging has demonstrated excellent capabilities in bioanalysis. However, the large size of the electrode and rapid diffusion of EFC molecules on the electrode surface have hindered imaging resolution and analytical performance. To overcome these challenges, bipolar nanoelectrodes (BPnE) array and in situ immobilization strategy of EFC molecules were proposed. The strategy achieved high spatial imaging resolution using anodized aluminum oxide (AAO) template-assisted Au nanoelectrodes array and avoided rapid diffusion of EFC molecules on the electrode surface by electrically polymerizing them on a single Au nanoelectrode. The proposed strategy exhibited ultra-high sensitivity for dopamine (DA) analysis and enabled monitoring of dynamic changes in DA release from living PC12 cells under drug stimulation.

CHINESE CHEMICAL LETTERS (2023)

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Article Chemistry, Analytical

Deep Learning Enhanced Electrochemiluminescence Microscopy

Pinlong Zhao et al.

Summary: A deep enhanced ECL microscopy (DEECL) method is proposed, which utilizes artificial neural networks to reconstruct electrochemiluminescence images with millisecond exposure times. DEECL achieves similar quality as high-quality electrochemiluminescence images with second-long exposure time. The DEECL method improves imaging efficiency by 1 to 2 orders than usual and achieves 85% accuracy in cell classification application.

ANALYTICAL CHEMISTRY (2023)

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Article Chemistry, Physical

Nanowire Array-based MOSFET for Future CMOS Technology to Attain the Ultimate Scaling Limit

Krutideepa Bhol et al.

Summary: Silicon nanowire (SiNW) structures play a crucial role in the field of electronic devices, with superior performance and potential. This paper investigates the performance of a silicon nanowire (Si NW) array-based GAA MOSFET using a quantum transport model, discussing its advantages and potential applications in lower technology nodes.

SILICON (2022)

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Article Chemistry, Analytical

Gold Nanowires Array-Based Closed Bipolar Nanoelectrode System for Electrochemiluminescence Detection of α-Fetoprotein on Cell Surface

Xiuxiu Li et al.

Summary: In this study, a gold nanowires array-based closed bipolar electrodes (c-BPEs) system was developed for sensitive detection of cell surface proteins. The system exhibited high sensitivity and was able to detect specific proteins at extremely low concentrations, as well as detect living cells through a synergistic amplification effect.

ANALYTICAL CHEMISTRY (2022)

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Article Biochemistry & Molecular Biology

Effects of Cations on HPTS Fluorescence and Quantification of Free Gadolinium Ions in Solution; Assessment of Intracellular Release of Gd3+ from Gd-Based MRI Contrast Agents

Angelo Scarciglia et al.

Summary: In this study, the fluorescence changes of 8-hydroxypyrene-1,3,6-trisulfonate (HPTS) under different conditions were investigated. The effects of mono-, di-, and trivalent cations on the fluorescence were analyzed, and a method for the quantification of trivalent cations was proposed. The study demonstrated the feasibility of a qualitative method to investigate the intracellular release of Gd3+ upon the administration of Gd-based contrast agents in murine macrophages.

MOLECULES (2022)

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Editorial Material Electrochemistry

Benzothioxanthene Dicarboximide as a Tuneable Electrogenerated Chemiluminescence Dye

Alice L. Dauphin et al.

CHEMELECTROCHEM (2022)

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Article Chemistry, Multidisciplinary

In Situ Imaging of Endogenous Hydrogen Peroxide Efflux from Living Cells via Bipolar Gold Nanoelectrode Array and Electrochemiluminescence Technology

Xiuxiu Li et al.

Summary: This study proposes a gold nanorod array based electrochemiluminescence system for in situ imaging of endogenous hydrogen peroxide (H2O2) released from living cells and detecting other molecules through cell surface modification. It has potential applications in cell apoptosis study and disease diagnosis.

ACS SENSORS (2022)

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Article Chemistry, Analytical

Wireless Imaging of Transient Redox Activity Based on Bipolar Light-Emitting Electrode Arrays

Gerardo Salinas et al.

Summary: Bipolar electrochemistry (BE) is a wireless electrochemical technique that utilizes an array of light-emitting diodes with different lengths to image the transient electrochemical activity of different redox species. The intensity of light emission allows differentiation of redox systems and evaluation of their diffusion behavior.

ANALYTICAL CHEMISTRY (2022)

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Article Biophysics

Bipolar silica nanochannel array confined electrochemiluminescence for ultrasensitive detection of SARS-CoV-2 antibody

Jiawei Gong et al.

Summary: In this study, a novel solid-state electrochemiluminescence (ECL) platform was developed for rapid and ultra-sensitive detection of SARS-CoV-2 antibody. The platform utilizes bipolar silica nanochannel array to stably confine the ECL probe and achieve reliable detection of SARS-CoV-2 IgG.

BIOSENSORS & BIOELECTRONICS (2022)

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Article Engineering, Biomedical

Super-Resolution Electrochemical Impedance Imaging With a 512 x 256 CMOS Sensor Array

Kangping Hu et al.

Summary: The research demonstrates the use of a CMOS sensor array for micro-scale super-resolution electrochemical impedance spectroscopy (SR-EIS) imaging. The sensor is designed to measure mutual capacitance between pixel pairs, allowing for the generation of spatially-resolved impedance images. Experimental results show the sensor's capability to produce microscale impedance images with sub-pixel resolution.

IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS (2022)

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Review Chemistry, Analytical

Advances in electrochemiluminescence for single-cell analysis

Qian Yang et al.

Summary: In recent years, innovative analytical methods with high sensitivity and spatiotemporal resolution have emerged for qualitative and quantitative analysis at the single-cell and subcellular levels. Among them, single-cell ECL (SCECL) analysis, with excellent spatiotemporal resolution, has become a promising tool for revealing individual cells' heterogeneity and stochastic processes.

ANALYST (2022)

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Article Chemistry, Multidisciplinary

Monitoring the electrochemical reactions on a gold nanoelectrode array via fluorescence-enabled electrochemical imaging

Xiuxiu Li et al.

Summary: High-spatial resolution fluorescence imaging on a high-density gold nanoelectrode array was achieved by anchoring fluorescent molecules to limit diffusion. The sensitivity of fluorescence imaging was effectively improved by using an ionic liquid with a refractive index matching the optical system. Spatially resolved imaging of electrochemical reactions on a single nanoelectrode modified with a single Pt nanoparticle was also demonstrated.

CHEMICAL COMMUNICATIONS (2022)

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Review Chemistry, Analytical

Recent Advancements in Bipolar Electrochemical Methods of Analysis

Kira L. Rahn et al.

ANALYTICAL CHEMISTRY (2021)

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Article Chemistry, Multidisciplinary

Single Biomolecule Imaging by Electrochemiluminescence

Yujie Liu et al.

Summary: This study successfully imaged single biomolecules using RuDSNs/AuNPs as ECL nanoemitters, demonstrating the visualization of single protein molecules and single membrane proteins in cells, overcoming the challenges in ultrasensitive ECL analysis and providing valuable information for cellular biology research.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2021)

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Article Electrochemistry

Electrocatalyst Screening on a Massive Array of Closed Bipolar Microelectrodes

Todd J. Anderson et al.

Summary: In this study, a novel electroanalytical platform utilizing bipolar ultramicroelectrode arrays and electrochemiluminescence imaging was developed for rapid screening of electrocatalysts. By electrodeposition of a radial gradient of catalytic metal on carbon-gold bipolar UME arrays, the impact of varying Ni(OH)2 coverage on the catalytic activity for the hydrogen evolution reaction (HER) was investigated. Future work will focus on expanding this platform to screen various metal alloys by incorporating multiple metal salts delivery during the gradient electrodeposition process.

JOURNAL OF THE ELECTROCHEMICAL SOCIETY (2021)

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Review Chemistry, Inorganic & Nuclear

Small molecular fluorescent probes for the detection of lead, cadmium and mercury ions

Shan-Yong Chen et al.

Summary: Heavy-metal pollution, especially Hg2+, Pb2+, and Cd2+, has become a serious global issue, threatening human health even at low concentrations. Developing rapid, accurate, and cost-effective detection methods is crucial in reducing exposure to these metals. Efforts have been focused on designing and synthesizing fluorescent probes for selective detection of Hg2+, Pb2+, and Cd2+.

COORDINATION CHEMISTRY REVIEWS (2021)

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Article Chemistry, Analytical

Electrofluorochromic Imaging Analysis of Glycan Expression on Living Single Cell with Bipolar Electrode Arrays

Zhaoyan Tian et al.

Summary: An integrated microfluidic system was designed for real-time monitoring of glycan expression on the surface of single tumor cells, achieving a level of 5.0 x 10^8 molecules per cell. This platform has the capability to distinguish single cancer cells from normal cells based on the expression level of glycans, providing a simple and accessible method for single-cell analysis.

ANALYTICAL CHEMISTRY (2021)

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Article Chemistry, Analytical

A Generalized Potentiostat Adaptor for Multiplexed Electroanalysis

Rebecca B. Clark et al.

Summary: Three different potentiostat architectures for electrochemical measurements are discussed, with a focus on the advantages of using a multiplexer interface. A strategy for developing flexible printed circuit (FPC) electrode arrays for single-channel potentiostats is presented, along with experimental results. The FPC array is interfaced with commercial potentiostats using a simple connector and multiplexers, demonstrating reliability, stability, and reproducibility.

ANALYTICAL CHEMISTRY (2021)

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Article Chemistry, Analytical

High Throughput Mapping of Single Molecules' Redox Potentials on Electrode

Chenghong Lei et al.

Summary: By synchronizing electrochemical potential scanning with a single-molecule localization super-resolution fluorescence microscope, this study tracked kinetic fluorescence changes of hundreds of single molecular redox events simultaneously with high throughput. Through subsequent cross-correlation function analysis, the redox potentials of single molecules were mapped out in unprecedented detail from site to site in the imaging area. This work lays the foundation for mapping redox states at single-molecule levels in high throughput in chemical and biological systems.

ANALYTICAL CHEMISTRY (2021)

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Article Engineering, Biomedical

An Integrated Biosensor System With a High-Density Microelectrode Array for Real-Time Electrochemical Imaging

William Tedjo et al.

IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS (2020)

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Article Chemistry, Analytical

Coupling electrochemistry with a fluorescence reporting reaction enabled by bipolar electrochemistry

Jessica S. Stefano et al.

JOURNAL OF ELECTROANALYTICAL CHEMISTRY (2020)

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Article Chemistry, Multidisciplinary

Closed Bipolar Electrode Array for On-Chip Analysis of Cellular Respiration by Cell Aggregates

Kosuke Ino et al.

ACS SENSORS (2020)

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Article Chemistry, Analytical

Electrochemiluminescence (ECL)-Based Electrochemical Imaging Using a Massive Array of Bipolar Ultramicroelectrodes

Todd J. Anderson et al.

ANALYTICAL CHEMISTRY (2020)

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Article Electrochemistry

Detection of Transient Nanoparticle Collision Events Using Electrochemiluminescence on a Closed Bipolar Microelectrode

Peter A. Defnet et al.

CHEMELECTROCHEM (2020)

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Article Multidisciplinary Sciences

Massively parallel microwire arrays integrated with CMOS chips for neural recording

Abdulmalik Obaid et al.

SCIENCE ADVANCES (2020)

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Article Chemistry, Analytical

Fabrication of High-Density and Superuniform Gold Nanoelectrode Arrays for Electrochemical Fluorescence Imaging

Xiang Qin et al.

ANALYTICAL CHEMISTRY (2020)

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Article Chemistry, Analytical

Visual Electrofluorochromic Detection of Cancer Cell Surface Glycoprotein on a Closed Bipolar Electrode Chip

Zhaoyan Tian et al.

ANALYTICAL CHEMISTRY (2019)

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Article Chemistry, Analytical

High-efficient preparation and screening of electrocatalysts using a closed bipolar electrode array system

Jia-Dong Zhang et al.

JOURNAL OF ELECTROANALYTICAL CHEMISTRY (2019)

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Article Chemistry, Analytical

In Situ Visualization of Electrocatalytic Reaction Activity at Quantum Dots for Water Oxidation

Ying Chen et al.

ANALYTICAL CHEMISTRY (2018)

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Article Biophysics

Electrochemical biosensor system using a CMOS microelectrode array provides high spatially and temporally resolved images

William Tedjo et al.

BIOSENSORS & BIOELECTRONICS (2018)

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Article Chemistry, Multidisciplinary

A snapshot of the electrochemical reaction layer by using 3 dimensionally resolved fluorescence mapping

Anne de Poulpiquet et al.

CHEMICAL SCIENCE (2018)

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Electrochemical detection of dopamine using periodic cylindrical gold nanoelectrode arrays

Da-Seul Kim et al.

SCIENTIFIC REPORTS (2018)

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Visualizing and Calculating Tip-Substrate Distance in Nanoscale Scanning Electrochemical Microscopy Using 3-Dimensional Super-Resolution Optical Imaging

Vignesh Sundaresan et al.

ANALYTICAL CHEMISTRY (2017)

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Imaging Electrode Heterogeneity Using Chemically Confined Fluorescence Electrochemical Microscopy

Jiratheep Pruchyathamkorn et al.

JOURNAL OF PHYSICAL CHEMISTRY LETTERS (2017)

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An RGB color-tunable turn-on electrofluorochromic device and its potential for information encryption

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Reaction Layer Imaging Using Fluorescence Electrochemical Microscopy

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Fluorescent Probes Used for Detection of Hydrogen Peroxide under Biological Conditions

Krzysztof Zamojc et al.

CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY (2016)

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Generation of electrochemiluminescence at bipolar electrodes: concepts and applications

Laurent Bouffier et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2016)

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Coupling Electrochemistry with Fluorescence Confocal Microscopy To Investigate Electrochemical Reactivity: A Case Study with the Resazurin-Resorufin Fluorogenic Couple

Thomas Doneux et al.

ANALYTICAL CHEMISTRY (2016)

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Fluorescence-Enabled Electrochemical Microscopy with Dihydroresorufin as a Fluorogenic Indicator

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Imaging Redox Activity at Bipolar Electrodes by Indirect Fluorescence Modulation

Laurent Bouffier et al.

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Imaging Transient Formation of Diffusion Layers with Fluorescence-Enabled Electrochemical Microscopy

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Highly durable colour/emission switching of fluorescein in a thin film device using electro-acid/base as in situ stimuli

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Fluorescence Coupling for Direct Imaging of Electrocatalytic Heterogeneity

Joshua P. Guerrette et al.

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Steady-State Voltammetry of a Microelectrode in a Closed Bipolar Cell

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