Optimization of obtaining extracts with photoprotective and antioxidant potential from Campomanesia adamantium (Cambess.) O. Berg

The species Campomanesia adamantium (Cambess.) O. Berg is native to the Brazilian Cerrado and has medicinal properties. Currently, researchers have been working on its domestication, bring-ing the possibility of its use in the elaboration of sustainable products. In this context, the objec-tive was to analyze the extraction yield at concentrations of 2% (m/v) and 5% (m/v) and the pho-toprotective effect of different extracts of C. adamantium leaves obtained with different concen-trations of ethanol and water, also, determine the preparation method with the greatest potential for phytocosmetic applications. Spectrophotometric methods were used to determine the levels of phenolic compounds, flavonoids, and antioxidant activity of the samples, as well as the sun protection factor (SPF), critical wavelength (lambda c), and UVA/UVB of the samples, besides the pro-file in the UV/Vis, infrared and UHPLC-DAD. The extraction concentration of 2% (m/v) showed yields significantly higher than 5% (m/v) by the independent t-test of the extracting solvent. In-frared scanning demonstrated the presence of functional groups associated with phenolic com-pounds. Through polynomial regression, it was found that ethanol concentrations between 75 and 100% are ideal for obtaining extracts rich in phenolic compounds, with antioxidant and pho-toprotective potential, but the concentration of 50% was more adequate to obtain a greater yield. Gallic acid, rutin, and 5,7-dihydroxy-6-methylflavanone were identified. All proportions of ethanol:water resulted in critical wavelength between 375 and 379 nm, and UVA/UVB ratio be-tween 1.17 +/- 0.01 and 1.53 +/- 0.07 which indicate a good distribution of UV absorption and possible photoprotection in the UVA region.

Automated summary

The study aimed to analyze the extraction yield and photoprotective effect of different extracts of Campomanesia adamantium leaves obtained with different concentrations of ethanol and water. The results showed that a 2% (m/v) extraction concentration had significantly higher yields than 5% (m/v). Ethanol concentrations between 75 and 100% were ideal for obtaining extracts rich in phenolic compounds with antioxidant and photoprotective potential. Gallic acid, rutin, and 5,7-dihydroxy-6-methylflavanone were identified in the extracts, and the extracts exhibited good UV absorption and potential photoprotection in the UVA region.