Synthesis and Application of Two-Photon Active Fluorescent Rhodol Dyes for Antibody Conjugation and In Vitro Cell Imaging

A novel family of julolidine-containing fluorescent rhodolsequippedwith a wide variety of substituents was synthesized in a versatiletwo-step process. The prepared compounds were fully characterizedand exhibited excellent fluorescence properties for microscopy imaging.The best candidate was conjugated to the therapeutic antibody trastuzumabthrough a copper-free strain-promoted azide-alkyne click reaction.The rhodol-labeled antibody was successfully applied for invitro confocal and two-photon microscopy imaging of Her2+cells.

Automated summary

A novel family of julolidine-containing fluorescent rhodolsequipped with a wide variety of substituents was synthesized using a versatile two-step process. The synthesized compounds were fully characterized and demonstrated excellent fluorescence properties for microscopy imaging. The best candidate was conjugated to the therapeutic antibody trastuzumab through a copper-free strain-promoted azide-alkyne click reaction. The rhodol-labeled antibody was successfully used for in vitro confocal and two-photon microscopy imaging of Her2+ cells.